Daily variations in the glycerol-3-phosphate dehydrogenase isoforms expression in Triatoma infestans flight muscles

Triatoma infestans, the main vector of Chagas disease, is a blood-sucking insect. Flight dispersal of adults is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in fligh...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2014-08, Vol.91 (2), p.399-405
Main Authors: Stroppa, María M, Carriazo, Carlota S, Gerez de Burgos, Nelia M, Garcia, Beatríz A
Format: Article
Language:English
Subjects:
Animals
Chagas Disease - transmission
Circadian Clocks - genetics
Female
Flight, Animal - physiology
Gene Expression Regulation
Glycerolphosphate Dehydrogenase - genetics
Glycerolphosphate Dehydrogenase - metabolism
Humans
Insect Proteins - genetics
Insect Proteins - metabolism
Insect Vectors
Isoenzymes - genetics
Isoenzymes - metabolism
Light
Male
Muscles - enzymology
Photoperiod
Transcription, Genetic
Triatoma - enzymology
Triatoma - genetics
Triatoma infestans
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Summary:Triatoma infestans, the main vector of Chagas disease, is a blood-sucking insect. Flight dispersal of adults is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. In this study, we explored the profile of GPDH expression in females and males adult flight muscles under light/dark cycle, constant light, and constant dark conditions. Under constant dark conditions, GPDH-1 flight muscles of T. infestans showed a rhythmic pattern of transcription synchronous with a rhythmic profile of activity suggesting regulation by the endogenous circadian clock. Otherwise, the GPDH-2 expression analysis showed no regulation by the endogenous clock, but showed that an external factor, such as the dark/light period, was necessary for synchronization of GPDH-2 transcription and activity.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.13-0556