Complete genome sequence and structural characterization of a novel iflavirus isolated from Opsiphanes invirae (Lepidoptera: Nymphalidae)

[Display omitted] •We characterized an iflavirus isolated from the palm oil lepidopterous pest, Opsiphanes invirae in Brazil.•This is the first report of iflavirus infecting O. invirae in Brazil.•This new virus (Opsiphanes invirae Iflavirus 1, OiIV-1) is more closely related to Spodoptera exigua ifl...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2015-09, Vol.130, p.136-140
Main Authors: Silva, Leonardo A., Ardisson-Araujo, Daniel M.P., Tinoco, Ricardo S., Fernandes, Odair A., Melo, Fernando L., Ribeiro, Bergmann M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological control
Elaeis guineensis
Genome sequencing
Genome, Viral - genetics
Iflavirus
Lepidoptera
Lepidoptera - virology
Microscopy, Electron, Transmission
Molecular Sequence Data
Nymphalidae
Opsiphanes invirae
Phylogeny
Picorna-like virus
Picornaviridae - physiology
Picornaviridae - ultrastructure
Spodoptera exigua
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Summary:[Display omitted] •We characterized an iflavirus isolated from the palm oil lepidopterous pest, Opsiphanes invirae in Brazil.•This is the first report of iflavirus infecting O. invirae in Brazil.•This new virus (Opsiphanes invirae Iflavirus 1, OiIV-1) is more closely related to Spodoptera exigua iflavirus 1 (SeIV-1). Opsiphanes invirae (Lepidopera: Nymphalidae) is a common pest of the African oil palm tree (Elaeis guineensis) in Brazil. Dead larvae were collected in canopy of oil palm trees cultivated in the amazon region (Para State) and analyzed for viral infection. Electron microscopy of caterpillar extracts showed an icosahedral picorna-like virus particle with 30nm in diameter. Total RNA extracted from partially purified virus particles was sequenced. A contig of 10,083 nucleotides (nt) was identified and showed to encode one single predicted polyprotein with 3185 amino acid residues. Phylogenetic analysis showed that the new virus was closely related to another lepidopteran infective virus Spodoptera exigua iflavirus 1(SeIV-1), with 35% amino acid pairwise identity. The novel virus fulfils all ICTV requirements for a new iflavirus species and was named Opsiphanes invirae Iflavirus 1 (OilV-1).
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2015.08.001