Serendipitous Meta-Transcriptomics: The Fungal Community of Norway Spruce (Picea abies): e0139080

After performing de novo transcript assembly of >1 billion RNA-Sequencing reads obtained from 22 samples of different Norway spruce (Picea abies) tissues that were not surface sterilized, we found that assembled sequences captured a mix of plant, lichen, and fungal transcripts. The latter were li...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9)
Main Authors: Delhomme, Nicolas, Sundstrom, Gorel, Zamani, Neda, Lantz, Henrik, Lin, Yao-Cheng, Hvidsten, Torgeir R, Hoppner, Marc P, Jern, Patric, Peer, Yves Vande, Lundeberg, Joakim
Format: Article
Language:English
Subjects:
Dothideomycetes
Picea abies
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:After performing de novo transcript assembly of >1 billion RNA-Sequencing reads obtained from 22 samples of different Norway spruce (Picea abies) tissues that were not surface sterilized, we found that assembled sequences captured a mix of plant, lichen, and fungal transcripts. The latter were likely expressed by endophytic and epiphytic symbionts, indicating that these organisms were present, alive, and metabolically active. Here, we show that these serendipitously sequenced transcripts need not be considered merely as contamination, as is common, but that they provide insight into the plant's phyllosphere. Notably, we could classify these transcripts as originating predominantly from Dothideomycetes and Leotiomycetes species, with functional annotation of gene families indicating active growth and metabolism, with particular regards to glucose intake and processing, as well as gene regulation.
ISSN:1932-6203
DOI:10.1371/journal.pone.0139080