Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation

Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs)...

Full description

Saved in:
Bibliographic Details
Published in:Biomacromolecules 2015-09, Vol.16 (9), p.2624-2630
Main Authors: Loebel, Claudia, Broguiere, Nicolas, Alini, Mauro, Zenobi-Wong, Marcy, Eglin, David
Format: Article
Language:English
Subjects:
Cells, Cultured
Cells, Immobilized - cytology
Cells, Immobilized - metabolism
Humans
Hyaluronic Acid - chemistry
Hydrogels - chemistry
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Oxidation-Reduction
Photochemical Processes
Photons
Tyramine - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93
cites cdi_FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93
container_end_page 2630
container_issue 9
container_start_page 2624
container_title Biomacromolecules
container_volume 16
creator Loebel, Claudia
Broguiere, Nicolas
Alini, Mauro
Zenobi-Wong, Marcy
Eglin, David
description Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs) with high viability. Macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2D and 3D microfabrication. Different degrees of cross-linking induce different swelling/shrinking, allowing for light-induced microactuation. These new tools are complementary to the previously reported horseradish peroxidase/hydrogen peroxide cross-linking and allow sequential cross-linking of HA-Tyr matrices.
doi_str_mv 10.1021/acs.biomac.5b00363
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1727693920</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1727693920</sourcerecordid><originalsourceid>FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93</originalsourceid><addsrcrecordid>eNp9kD1PwzAYhC0EoqXwBxiQRxYX24ntZEQVUKSiIlHmyHac1iWxi90I8u9JP2Bkek967066B4BrgscEU3IndRwr6xupx0xhnPDkBAwJoxylHNPTvWZIiFwMwEWMa4xxnqTsHAwop5QSmg3B6sXq4CupgtVya72DvoKvK7_1aBJ8jGhm3Ycp4bSTdRu8k66XZfBLU0eoOvhm3bI2CEpXwsWXR_uog4suyMY6A-ffttz3XoKzStbRXB3vCLw_PiwmUzSbPz1P7mdIpinZolQRlXGRKE0V5lRyymiWZVJyVTFMdCWkYFyTLKdEJ8QIhjPW_3cfwas8GYHbQ-8m-M_WxG3R2KhNXUtnfBsLIqjgeZJT3Fvpwap3S4Opik2wjQxdQXCxI1z0hIsD4eJIuA_dHPtb1ZjyL_KLtDeMD4ZdeO3b4Pq5_zX-ANAliSI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1727693920</pqid></control><display><type>article</type><title>Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read &amp; Publish Agreement 2022-2024 (Reading list)</source><creator>Loebel, Claudia ; Broguiere, Nicolas ; Alini, Mauro ; Zenobi-Wong, Marcy ; Eglin, David</creator><creatorcontrib>Loebel, Claudia ; Broguiere, Nicolas ; Alini, Mauro ; Zenobi-Wong, Marcy ; Eglin, David</creatorcontrib><description>Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs) with high viability. Macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2D and 3D microfabrication. Different degrees of cross-linking induce different swelling/shrinking, allowing for light-induced microactuation. These new tools are complementary to the previously reported horseradish peroxidase/hydrogen peroxide cross-linking and allow sequential cross-linking of HA-Tyr matrices.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/acs.biomac.5b00363</identifier><identifier>PMID: 26222128</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Cells, Cultured ; Cells, Immobilized - cytology ; Cells, Immobilized - metabolism ; Humans ; Hyaluronic Acid - chemistry ; Hydrogels - chemistry ; Materials Testing ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Oxidation-Reduction ; Photochemical Processes ; Photons ; Tyramine - chemistry</subject><ispartof>Biomacromolecules, 2015-09, Vol.16 (9), p.2624-2630</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93</citedby><cites>FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26222128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loebel, Claudia</creatorcontrib><creatorcontrib>Broguiere, Nicolas</creatorcontrib><creatorcontrib>Alini, Mauro</creatorcontrib><creatorcontrib>Zenobi-Wong, Marcy</creatorcontrib><creatorcontrib>Eglin, David</creatorcontrib><title>Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs) with high viability. Macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2D and 3D microfabrication. Different degrees of cross-linking induce different swelling/shrinking, allowing for light-induced microactuation. These new tools are complementary to the previously reported horseradish peroxidase/hydrogen peroxide cross-linking and allow sequential cross-linking of HA-Tyr matrices.</description><subject>Cells, Cultured</subject><subject>Cells, Immobilized - cytology</subject><subject>Cells, Immobilized - metabolism</subject><subject>Humans</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hydrogels - chemistry</subject><subject>Materials Testing</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Photochemical Processes</subject><subject>Photons</subject><subject>Tyramine - chemistry</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAYhC0EoqXwBxiQRxYX24ntZEQVUKSiIlHmyHac1iWxi90I8u9JP2Bkek967066B4BrgscEU3IndRwr6xupx0xhnPDkBAwJoxylHNPTvWZIiFwMwEWMa4xxnqTsHAwop5QSmg3B6sXq4CupgtVya72DvoKvK7_1aBJ8jGhm3Ycp4bSTdRu8k66XZfBLU0eoOvhm3bI2CEpXwsWXR_uog4suyMY6A-ffttz3XoKzStbRXB3vCLw_PiwmUzSbPz1P7mdIpinZolQRlXGRKE0V5lRyymiWZVJyVTFMdCWkYFyTLKdEJ8QIhjPW_3cfwas8GYHbQ-8m-M_WxG3R2KhNXUtnfBsLIqjgeZJT3Fvpwap3S4Opik2wjQxdQXCxI1z0hIsD4eJIuA_dHPtb1ZjyL_KLtDeMD4ZdeO3b4Pq5_zX-ANAliSI</recordid><startdate>20150914</startdate><enddate>20150914</enddate><creator>Loebel, Claudia</creator><creator>Broguiere, Nicolas</creator><creator>Alini, Mauro</creator><creator>Zenobi-Wong, Marcy</creator><creator>Eglin, David</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20150914</creationdate><title>Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation</title><author>Loebel, Claudia ; Broguiere, Nicolas ; Alini, Mauro ; Zenobi-Wong, Marcy ; Eglin, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cells, Cultured</topic><topic>Cells, Immobilized - cytology</topic><topic>Cells, Immobilized - metabolism</topic><topic>Humans</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hydrogels - chemistry</topic><topic>Materials Testing</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Photochemical Processes</topic><topic>Photons</topic><topic>Tyramine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loebel, Claudia</creatorcontrib><creatorcontrib>Broguiere, Nicolas</creatorcontrib><creatorcontrib>Alini, Mauro</creatorcontrib><creatorcontrib>Zenobi-Wong, Marcy</creatorcontrib><creatorcontrib>Eglin, David</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loebel, Claudia</au><au>Broguiere, Nicolas</au><au>Alini, Mauro</au><au>Zenobi-Wong, Marcy</au><au>Eglin, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2015-09-14</date><risdate>2015</risdate><volume>16</volume><issue>9</issue><spage>2624</spage><epage>2630</epage><pages>2624-2630</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>Photo-cross-linking of tyramine-substituted hyaluronan (HA-Tyr) hydrogels is demonstrated for the first time. HA-Tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. Nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hMSCs) with high viability. Macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2D and 3D microfabrication. Different degrees of cross-linking induce different swelling/shrinking, allowing for light-induced microactuation. These new tools are complementary to the previously reported horseradish peroxidase/hydrogen peroxide cross-linking and allow sequential cross-linking of HA-Tyr matrices.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26222128</pmid><doi>10.1021/acs.biomac.5b00363</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1525-7797
ispartof Biomacromolecules, 2015-09, Vol.16 (9), p.2624-2630
issn 1525-7797
1526-4602
language eng
recordid cdi_proquest_miscellaneous_1727693920
source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Cells, Cultured
Cells, Immobilized - cytology
Cells, Immobilized - metabolism
Humans
Hyaluronic Acid - chemistry
Hydrogels - chemistry
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Oxidation-Reduction
Photochemical Processes
Photons
Tyramine - chemistry
title Microfabrication of Photo-Cross-Linked Hyaluronan Hydrogels by Single- and Two-Photon Tyramine Oxidation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T11%3A50%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microfabrication%20of%20Photo-Cross-Linked%20Hyaluronan%20Hydrogels%20by%20Single-%20and%20Two-Photon%20Tyramine%20Oxidation&rft.jtitle=Biomacromolecules&rft.au=Loebel,%20Claudia&rft.date=2015-09-14&rft.volume=16&rft.issue=9&rft.spage=2624&rft.epage=2630&rft.pages=2624-2630&rft.issn=1525-7797&rft.eissn=1526-4602&rft_id=info:doi/10.1021/acs.biomac.5b00363&rft_dat=%3Cproquest_cross%3E1727693920%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a441t-4b1b8673bc2b062a6252888aa6bf501cf7a756c18921c31e7508588a1cf776f93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1727693920&rft_id=info:pmid/26222128&rfr_iscdi=true