Flavobacterium notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng

• Published in: Antonie van Leeuwenhoek 2015-09, Vol.108 (3), p.545-552
• Main Authors: Zhang, Meng-Yue, Xu, Huan, Zhang, Tian-Yuan, Xie, Jun, Cheng, Juan, Nimaichand, Salam, Li, Shan-Hui, Li, Wen-Jun, Zhang, Yi-Xuan
• Format: Article
• Language: English
• Subjects: Aerobiosis; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; China; Cluster Analysis; color; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; Flavobacterium; Flavobacterium - classification; Flavobacterium - genetics; Flavobacterium - isolation & purification; Flavobacterium - physiology; Genes; Hydrogen-Ion Concentration; Life Sciences; Lipids; Medical Microbiology; Microbiology; Microscopy, Electron, Transmission; Molecular Sequence Data; new species; Nucleic Acid Hybridization; nucleotide sequences; Original Paper; Panax; Panax notoginseng; Panax notoginseng - growth & development; phosphatidylethanolamines; Phospholipids - analysis; Phylogeny; Pigments, Biological - metabolism; Plant Sciences; Quinones - analysis; Rhizosphere; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; soil; Soil Microbiology; Soil Science & Conservation; Temperature
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-015-0509-0

Two novel bacterial strains, designated SYP-B540ᵀ and SYP-B556, were isolated from rhizospheric soil of Panax notoginseng located at Yunnan Province, China. Both strains were Gram-staining negative, aerobic, non-motile, elongated rod shaped and yellow coloured. They grew optimally at 28 °C and pH 7.0. Analysis of 16S rRNA gene sequences showed that the two strains shared 99.8 % sequence similarity to each other, but lower than 97.6 % to the other known species of the genus Flavobacterium. The predominant respiratory quinone for the two strains was MK-6, and the major fatty acids were iso-C₁₅:₀ and summed Feature 3 (comprising 16:1 ω7c and/or 16:1 ω6c). The polar lipids consisted of phosphatidylethanolamine, two unidentified polar lipids and three unidentified amino-phospholipids. The DNA G+C contents of strains SYP-B540ᵀ and SYP-B556 were 33.3 and 32.7 mol%, respectively. In addition, the DNA–DNA hybridization values of strains SYP-B540ᵀ and SYP-B556 to their closest phylogenetic neighbors were significantly lower than 70 %. On the basis of the polyphasic taxonomy studies, strains SYP-B540ᵀ and SYP-B556 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium notoginsengisoli sp. nov. is proposed. The type strain is SYP-B540ᵀ (=KCTC 32505ᵀ = NBRC 110012ᵀ = BCRC 80724ᵀ).