Some properties and possible biological role of peptidase inhibitors from the entomopathogenic fungus Tolypocladium cylindrosporum

• Published in: Archives of microbiology 2015-10, Vol.197 (8), p.1001-1010
• Main Authors: Popova, V. V, Dunaevsky, Y. E, Domash, V. I, Semenova, T. A, Beliakova, G. A, Belozersky, M. A
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; antibiotics; bacteria; Bacterial Proteins - metabolism; Biochemistry; Biomedical and Life Sciences; Biotechnology; bromelains; Cell Biology; Dengue fever; Ecology; entomopathogenic fungi; Enzyme Activation - drug effects; Enzymes; Enzymes - metabolism; Fungi; Hypocreales; Hypocreales - chemistry; Inhibitors; Insects; Investigations; Life Sciences; Metabolism; Microbial Ecology; Microbiology; molecular weight; mycelium; Organisms; Original Paper; papain; Pathogenesis; Peptides; Protease Inhibitors - isolation & purification; Protease Inhibitors - pharmacology; Proteins; proteolysis; Pseudomonas; Pseudomonas - drug effects; subtilisin; Tolypocladium; Tolypocladium cylindrosporum; Tropical diseases; trypsin
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-015-1132-1

The activities of secreted and mycelial inhibitors of proteolytic enzymes from fungi of the order Hypocreales have been investigated. Inhibitors of bromelain, papain, and trypsin of low molecular mass (about 1 kDa) and a subtilisin proteinaceous inhibitor with molecular mass of 45 kDa were revealed in the culture liquid of the fungus Tolypocladium cylindrosporum. The subtilisin inhibitor from T. cylindrosporum has antibiotic properties, significantly decreased the activity of purified bacterial enzymes, and prevented the growth of the bacterium Pseudomonas sp. Data suggesting the existence in fungi of the Hypocreales order of two pools of peptidase inhibitors have been obtained.