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Phosphoenolpyruvate carboxykinase (PEPCK) deficiency affects the germination, growth and fruit sugar content in tomato (Solanum lycopersicum L.)

Phosphoenolpyruvate carboxykinase (PEPCK) is a key regulatory enzyme and is utilized in the gluconeogenesis pathway in plants. Although, its catalytic and regulatory properties are quite well understood, there are uncertainties regarding its physiological role in many plants tissues such as the fles...

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Published in:Plant physiology and biochemistry 2015-11, Vol.96, p.417-425
Main Authors: Huang, Yong-Xing, Yin, Yong-Gen, Sanuki, Atsuko, Fukuda, Naoya, Ezura, Hiroshi, Matsukura, Chiaki
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container_title Plant physiology and biochemistry
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description Phosphoenolpyruvate carboxykinase (PEPCK) is a key regulatory enzyme and is utilized in the gluconeogenesis pathway in plants. Although, its catalytic and regulatory properties are quite well understood, there are uncertainties regarding its physiological role in many plants tissues such as the flesh of developing fruits. To further understand the function of PEPCK in fruits and other tissues, RNAi transgenic tomato plants in which SlPEPCK transcription was down-regulated by either CaMV 35S constitutive promoter or the fruit-specific E8 promoter were generated and characterized on the basis of their phenotypic and metabolic aspects. In the PEPCK-deficient lines, prominent growth suppression of germinated seedlings was observed and other vegetative suppression appeared during the early stage of plant growth in the 35S promoter-driven lines. In particular, root elongation was most obviously suppressed in the germinated seedlings, indicating that the gluconeogenesis pathway is involved in the root growth of seedlings. Regarding the primary metabolism in fruit, the soluble sugar content tended to decrease, whereas the malate content tended to increase in ripening fruits of the RNAi lines compared with the wild type. These results indicate that activation of the gluconeogenesis pathway from organic acids to sugars occurs during ripening but is suppressed by the knocking down of the PEPCK gene, suggesting that PEPCK participates in determining the sugar/acid ratio in ripening fruit. •Systemic PEPCK suppression caused remarkable growth suppression in seedlings.•Systemic PEPCK suppression caused growth suppression in juvenile plants.•Soluble sugar contents decreased in RNAi transgenic fruit.•Malate content showed an increasing tendency in RNAi transgenic fruit.
doi_str_mv 10.1016/j.plaphy.2015.08.021
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Regarding the primary metabolism in fruit, the soluble sugar content tended to decrease, whereas the malate content tended to increase in ripening fruits of the RNAi lines compared with the wild type. These results indicate that activation of the gluconeogenesis pathway from organic acids to sugars occurs during ripening but is suppressed by the knocking down of the PEPCK gene, suggesting that PEPCK participates in determining the sugar/acid ratio in ripening fruit. •Systemic PEPCK suppression caused remarkable growth suppression in seedlings.•Systemic PEPCK suppression caused growth suppression in juvenile plants.•Soluble sugar contents decreased in RNAi transgenic fruit.•Malate content showed an increasing tendency in RNAi transgenic fruit.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>26381194</pmid><doi>10.1016/j.plaphy.2015.08.021</doi><tpages>9</tpages></addata></record>
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subjects Carbohydrate Metabolism
Fruit
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Germination - physiology
Gluconeogenesis
Lycopersicon esculentum - enzymology
Lycopersicon esculentum - growth & development
Lycopersicon esculentum - metabolism
Lycopersicon esculentum - physiology
PEPCK
Phosphoenolpyruvate Carboxykinase (ATP) - genetics
Phosphoenolpyruvate Carboxykinase (ATP) - metabolism
Phosphoenolpyruvate Carboxykinase (ATP) - physiology
Plants, Genetically Modified
Seedling growth
Sugar content
Tomato
Transcription, Genetic
title Phosphoenolpyruvate carboxykinase (PEPCK) deficiency affects the germination, growth and fruit sugar content in tomato (Solanum lycopersicum L.)
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