Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem: a proposed methodology for germplasm collection

Five arbuscular mycorrhizal (AM) fungi, isolated from the rhizosphere of banana and sugar cane, were successfully cultured in vitro in association with genetically transformed roots of carrot. The intraradical forms of the fungi as mycorrhizal root pieces and single isolated vesicles constituted exc...

Full description

Saved in:
Bibliographic Details
Published in:Mycologia 1998-07, Vol.90 (4), p.579-585
Main Authors: Declerck, S. (Universite Catholique de Louvain, Louvain-La-Neuve, Belgium.), Strullu, D.G, Plenchette, C
Format: Article
Language:English
Subjects:
arbuscular mycorrhizal fungi
Carrots
continuous culture
CRECIMIENTO
CROISSANCE
CULTURE TECHNIQUES
DAUCUS CAROTA
Ecology
fungal library
Fungal spores
Fungi
GERMOPLASMA
GERMPLASM
GLOMUS
GLOMUS FASCICULATUM
GLOMUS MACROCARPUM
GLOMUS VERSIFORME
GROWTH
Hyphae
Inoculum
ISOLATION TECHNIQUES
Leeks
Life Sciences
MICORRIZAS ARBUSCULARES VESICULARES
Microbiology and Parasitology
MUSA
Mycelium
Mycology
MYCORHIZE A VESICULE ET ARBUSCULE
Mycorrhizal fungi
Ri T-DNA transformed root
SACCHARUM
SIMBIOSIS
Subcultures
SYMBIOSE
SYMBIOSIS
TECHNIQUE DE CULTURE
TECHNIQUE DE L'ISOLEMENT
TECNICAS DE AISLAMIENTO
TECNICAS DE CULTIVO
vesicle
VESICULAR ARBUSCULAR MYCORRHIZAE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Five arbuscular mycorrhizal (AM) fungi, isolated from the rhizosphere of banana and sugar cane, were successfully cultured in vitro in association with genetically transformed roots of carrot. The intraradical forms of the fungi as mycorrhizal root pieces and single isolated vesicles constituted excellent sources of inoculum for the establishment of in vitro cultures and for the continuous culture of the species. Several thousand fungal propagules were obtained for the two G. versiforme species, G. intraradices and to a lesser extent for G. fasciculatum whereas few spores were obtained for G. macrocarpum. This coculture system appeared suitable for the establishment of in vitro collections of AM fungal strains.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1998.12026946