Genotypic variation of the response to cadmium toxicity in Pisum sativum L

• Published in: Journal of experimental botany 2005-01, Vol.56 (409), p.167-178
• Main Authors: Metwally, A, Safronova, V.I, Belimov, A.A, Dietz, K.J
• Format: Article
• Language: English
• Subjects: abiotic stress; Ascorbate peroxidase; Ascorbate Peroxidases; Cadmium; Cadmium - metabolism; Cadmium - toxicity; catalase; chemical constituents of plants; chitinase; Chitinases - metabolism; enzyme activity; Enzymes; field crops; genetic variation; Genotype; Genotypes; glutathione; Glutathione - metabolism; Heavy metals; Hydroponics; Lipid Peroxidation - physiology; malondialdehyde; Malondialdehyde - metabolism; metal tolerance; nutrient uptake; Oxidative stress; Peas; peroxidase; peroxidases; Peroxidases - metabolism; phytotoxicity; Pisum sativum; Pisum sativum - drug effects; Pisum sativum - genetics; Pisum sativum - growth & development; Pisum sativum - metabolism; Pisum sativum L; Plant biochemistry; plant nutrition; Plant roots; Plant Roots - drug effects; Plants; RESEARCH PAPER; Sulfhydryl Compounds - metabolism; thiols
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/eri017

This work evaluates the (cor-)relations between selected biochemical responses to toxic Cd and the degree of Cd sensitivity in a set of pea genotypes. Ten genotypes were analysed that differ in their growth response to Cd when expressed as root or shoot tolerance indices (TIs). Concentrations of non-protein thiols (NPTs) and malondialdehyde (MDA), activity of chitinase, peroxidase (POX), and catalase significantly increased in all pea genotypes treated with Cd. Cd-sensitivity of genotypes was correlated with relative increases in MDA concentration as well as activities of chitinase and POX, suggesting similar Cd stress effects. Activities of ascorbate peroxidase (APX) decreased, but concentrations of glutathione (GSH) increased in the less Cd-sensitive genotypes. Differences in root and leaf contents of Cd revealed no correlation with TI, metabolic parameters, and enzyme activities in Cd-treated plants, respectively, except that shoot Cd concentration positively correlated with shoot chitinase activity. Toxic Cd levels inhibited uptake of nutrient elements such as P, K, S, Ca, Zn, Mn, and B by plants in an organ- and genotype-specific manner. Cd-sensitivity was significantly correlated with decreased root Zn concentrations. The results show both similarities, as well as distinct features, in Cd toxicity expression in genotypes of one species, suggesting that independent and multi-factorial reactions modulate Cd sensitivity on the low-tolerance level of plants. The study illustrates the biochemical basis of earlier detected genotypic variation in Cd response.