Identification of domains involved in superantigenicity of streptococcal pyrogenic exotoxin F (SpeF)

A series of 11 synthetic peptides of 30 amino acids, each with 10 amino acids overlap which spanned the entire sequence of streptococcal pyrogenic exotoxin F (SpeF), were employed in proliferation studies on human peripheral blood mononuclear cells (PBMCs). Regions 41–70, 141–170 and 181–210 were id...

Full description

Saved in:
Bibliographic Details
Published in:Microbial pathogenesis 1998-11, Vol.25 (5), p.279-290
Main Authors: Eriksson, Anna, Holm, Stig E, Norgren, Mari
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - chemistry
Amino Acid Sequence
Animals
Antibodies, Bacterial - biosynthesis
Bacterial Proteins
Bacteriology
Biological and medical sciences
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Exotoxins - chemistry
Exotoxins - immunology
Fundamental and applied biological sciences. Psychology
Group A streptococci, superantigen, SpeF, mitogenic epitopes, synthetic peptides
Hemocyanins - chemistry
Lymphocyte Activation
Membrane Proteins
Microbiology
Molecular Sequence Data
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peptides - chemical synthesis
Protein Structure, Secondary
Pyrogens - chemistry
Pyrogens - immunology
Rabbits
Streptococcal Infections - immunology
Streptococcus
Streptococcus pyogenes - chemistry
Streptococcus pyogenes - immunology
Superantigens - chemistry
Superantigens - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A series of 11 synthetic peptides of 30 amino acids, each with 10 amino acids overlap which spanned the entire sequence of streptococcal pyrogenic exotoxin F (SpeF), were employed in proliferation studies on human peripheral blood mononuclear cells (PBMCs). Regions 41–70, 141–170 and 181–210 were identified as important for SpeF-induced lymphocyte activation. Secondary structure predictions of these peptides showed similarities to regions in other superantigens known to be important for T cell mitogenicity. Furthermore, antisera specific to peptides covering amino acids 1–70 and 181–228 were able to inhibit SpeF-induced mitogenicity by 25% when pre-incubated with SpeF prior to PBMC activation.
ISSN:0882-4010
1096-1208
DOI:10.1006/mpat.1998.0234