Nucleic acid (DNA, RNA) quantification and RNA/DNA ratio determination in marine sediments : Comparison of spectrophotometric, fluorometric, and high-performance liquid chromatography methods and estimation of detrital DNA

In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for...

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Bibliographic Details
Published in:Applied and environmental microbiology 1998-09, Vol.64 (9), p.3238-3245
Main Authors: DELL'ANNO, A, FABIANO, M, DUINEVELD, G. C. A, KOK, A, DANOVARO, R
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Biological and medical sciences
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Marine biology
Metabolism
Microbial ecology
Microbiology
Ribonucleic acid
RNA
Sediments
Various environments (extraatmospheric space, air, water)
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Summary:In this study, we compared three methods for extraction and quantification of RNA and DNA from marine sediments: (i) a spectrophotometric method using the diphenylamine assay; (ii) a fluorometric method utilizing selective fluorochromes (thiazole orange for total nucleic acids and Hoechst 33258 for DNA); and (iii) a high-pressure liquid chromatography (HPLC) method which uses a specific column to separate RNA and DNA and UV absorption of the nucleic acids for quantification.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.64.9.3238-3245.1998