Production of glucosyltransferases by wild-type Leuconostoc mesenteroides in media containing sugars other than sucrose

• Published in: Journal of industrial microbiology & biotechnology 1999-03, Vol.22 (3), p.139-146
• Main Authors: SMITH, M. R, ZAHNLEY, J. C
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biosynthesis; Biotechnology; Enzyme engineering; Enzymes; Fermentation; Fundamental and applied biological sciences. Psychology; Glucose; Leuconostoc mesenteroides; Methods. Procedures. Technologies; Production of selected enzymes; Proteins; Reagents; Sodium; Studies; Sucrose; Sugar
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1038/sj.jim.2900621

produces glucosyltransferases (GTFs) and fructosyltransferases (FTFs) which are inducible enzymes which respectively synthesize dextrans and levans from sucrose. Except for a few mutant strains which produce high activities in glucose medium, L. mesenteroides is thought not to produce GTFs and FTFs unless sucrose is present. We show here that cultures of eight strains produced low, but detectable GTF activity when glucose, maltose or melibiose replaced sucrose as the growth substrate. Four strains also produced FTFs of approximately 130kDa in medium with or without sucrose. The GTFs and FTFs produced on sugars other than sucrose could be detected as bands on SDS gels even when not detected by other methods. Except for strain B-523, the number, sizes and relative intensities of the bands were independent of the sugar used for growing the cultures. Alternansucrase from strains B-1355 and B-1501 in glucose or maltose medium was almost entirely associated with the cell fraction, ruling out binding to glucans as the cause of the association.[PUBLICATION ABSTRACT]