Culturing and bioassay testing of Daphnia magna using Elendt M4, Elendt M7, and COMBO media

A performance-based, tiered approach was used to evaluate survival, growth, and reproduction of Daphnia magna in three selected synthetic media: Elendt M4, Elendt M7, and COMBO. Both Elendt M4 and Elendt M7 are high-hardness media currently recommended for Organization for Economic Cooperation and D...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety 1999-05, Vol.43 (1), p.103-110
Main Authors: SAMEL, A, ZIEGENFUSS, M, GOULDEN, C. E, BANKS, S, BAER, K. N
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Biological Assay - methods
Culture Media - chemical synthesis
Daphnia - drug effects
Daphnia - growth & development
Daphnia - metabolism
Daphnia - physiology
Daphnia magna
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
Fundamental and applied biological sciences. Psychology
Toxicology - methods
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Summary:A performance-based, tiered approach was used to evaluate survival, growth, and reproduction of Daphnia magna in three selected synthetic media: Elendt M4, Elendt M7, and COMBO. Both Elendt M4 and Elendt M7 are high-hardness media currently recommended for Organization for Economic Cooperation and Development (OECD) testing. COMBO is a softer medium similar in total hardness to natural water found in the environment. Tier I consistent of an acclimation phase; Tier II involved a 21-day evaluation of survival, growth, and reproduction; and Tier III used each medium in a 21-day chronic study using a reference toxicant, 3,4-dichloroaniline (3,4-DCA). The evaluation of the performance of each medium was based on acceptance criteria similar to those used by the U.S. Environmental Protection Agency and the European Commission. Tests were run concurrently at three laboratories to assess interlaboratory variability. Daphnids were acclimated to the media for less than 1 month. Daphnid performance in all media exceeded the European Economic Community (EEC) validity criteria; however, reproductive performance and growth were significantly greater in the Elendt media than in COMBO. 3,4-DCA exerted more toxicity to daphnids in COMBO medium [no-observed-effect concentration (NOEC),
ISSN:0147-6513
1090-2414
DOI:10.1006/eesa.1999.1777