A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application

A pyrene-based fluorescent sensor ( PHP ) was synthesized for Cu( ii ) detection. It had high selectivity towards Cu 2+ ions via photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu 2+ , PHP provided significant blue emission, while Ag + , Al 3+ , Ca 2+ , Cd 2+...

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Bibliographic Details
Published in:RSC advances 2015-01, Vol.5 (53), p.42591-42596
Main Authors: Venkatesan, Parthiban, Wu, Shu-Pao
Format: Article
Language:English
Subjects:
Binding
Chemoreceptors
Confocal
Constants
Emission
Fluorescence
Microscopy
Spectral emissivity
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Summary:A pyrene-based fluorescent sensor ( PHP ) was synthesized for Cu( ii ) detection. It had high selectivity towards Cu 2+ ions via photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu 2+ , PHP provided significant blue emission, while Ag + , Al 3+ , Ca 2+ , Cd 2+ , Co 2+ , Cr 3+ , Fe 2+ , Fe 3+ , Hg 2+ , Mg 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ metal ions produced only minor changes in the fluorescence spectra. The association constant ( K a ) for Cu 2+ binding to PHP had a value of 1.0 × 10 4 M −1 . The maximum emission change induced by Cu 2+ binding to the chemosensor PHP was observed over the pH range 5.0–10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that PHP can be used as an effective fluorescent probe for detecting Cu 2+ in living cells.
ISSN:2046-2069
2046-2069
DOI:10.1039/C5RA05440K