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A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application
A pyrene-based fluorescent sensor ( PHP ) was synthesized for Cu( ii ) detection. It had high selectivity towards Cu 2+ ions via photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu 2+ , PHP provided significant blue emission, while Ag + , Al 3+ , Ca 2+ , Cd 2+...
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| Published in: | RSC advances 2015-01, Vol.5 (53), p.42591-42596 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | 42596 |
| container_issue | 53 |
| container_start_page | 42591 |
| container_title | RSC advances |
| container_volume | 5 |
| creator | Venkatesan, Parthiban Wu, Shu-Pao |
| description | A pyrene-based fluorescent sensor (
PHP
) was synthesized for Cu(
ii
) detection. It had high selectivity towards Cu
2+
ions
via
photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu
2+
,
PHP
provided significant blue emission, while Ag
+
, Al
3+
, Ca
2+
, Cd
2+
, Co
2+
, Cr
3+
, Fe
2+
, Fe
3+
, Hg
2+
, Mg
2+
, Mn
2+
, Ni
2+
, Pb
2+
, and Zn
2+
metal ions produced only minor changes in the fluorescence spectra. The association constant (
K
a
) for Cu
2+
binding to
PHP
had a value of 1.0 × 10
4
M
−1
. The maximum emission change induced by Cu
2+
binding to the chemosensor
PHP
was observed over the pH range 5.0–10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that
PHP
can be used as an effective fluorescent probe for detecting Cu
2+
in living cells. |
| doi_str_mv | 10.1039/C5RA05440K |
| format | article |
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PHP
) was synthesized for Cu(
ii
) detection. It had high selectivity towards Cu
2+
ions
via
photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu
2+
,
PHP
provided significant blue emission, while Ag
+
, Al
3+
, Ca
2+
, Cd
2+
, Co
2+
, Cr
3+
, Fe
2+
, Fe
3+
, Hg
2+
, Mg
2+
, Mn
2+
, Ni
2+
, Pb
2+
, and Zn
2+
metal ions produced only minor changes in the fluorescence spectra. The association constant (
K
a
) for Cu
2+
binding to
PHP
had a value of 1.0 × 10
4
M
−1
. The maximum emission change induced by Cu
2+
binding to the chemosensor
PHP
was observed over the pH range 5.0–10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that
PHP
can be used as an effective fluorescent probe for detecting Cu
2+
in living cells.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/C5RA05440K</identifier><language>eng</language><subject>Binding ; Chemoreceptors ; Confocal ; Constants ; Emission ; Fluorescence ; Microscopy ; Spectral emissivity</subject><ispartof>RSC advances, 2015-01, Vol.5 (53), p.42591-42596</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-476576fe7f781a4d1e0567f9794c0f42badca4e695a61ceb77fc4a309e3094173</citedby><cites>FETCH-LOGICAL-c264t-476576fe7f781a4d1e0567f9794c0f42badca4e695a61ceb77fc4a309e3094173</cites><orcidid>0000-0002-4424-5486</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Venkatesan, Parthiban</creatorcontrib><creatorcontrib>Wu, Shu-Pao</creatorcontrib><title>A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application</title><title>RSC advances</title><description>A pyrene-based fluorescent sensor (
PHP
) was synthesized for Cu(
ii
) detection. It had high selectivity towards Cu
2+
ions
via
photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu
2+
,
PHP
provided significant blue emission, while Ag
+
, Al
3+
, Ca
2+
, Cd
2+
, Co
2+
, Cr
3+
, Fe
2+
, Fe
3+
, Hg
2+
, Mg
2+
, Mn
2+
, Ni
2+
, Pb
2+
, and Zn
2+
metal ions produced only minor changes in the fluorescence spectra. The association constant (
K
a
) for Cu
2+
binding to
PHP
had a value of 1.0 × 10
4
M
−1
. The maximum emission change induced by Cu
2+
binding to the chemosensor
PHP
was observed over the pH range 5.0–10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that
PHP
can be used as an effective fluorescent probe for detecting Cu
2+
in living cells.</description><subject>Binding</subject><subject>Chemoreceptors</subject><subject>Confocal</subject><subject>Constants</subject><subject>Emission</subject><subject>Fluorescence</subject><subject>Microscopy</subject><subject>Spectral emissivity</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpNkFFLwzAUhYMoOOZe_AV5nEL1pk2T5XEUneJAEPW1pNkNi3RJTVpl_96OCXrhcM_Dx-FwCLlkcMOgULdV-bKEknN4OiGTHLjIchDq9J8_J7OUPmA8UbJcsAl5X9J-iD4Lntp2CBGTQd_Tbh_RY9bohBtqtrgLCX0KkdpR1TCnztEr-u36LW3dF1KDbUt117XO6N4Ff0HOrG4Tzn7_lLzd371WD9n6efVYLdeZyQXvMy5FKYVFaeWCab5hCKWQVknFDVieN3pjNEehSi2YwUZKa7guQOEozmQxJfNjbhfD54Cpr3cuHcpoj2FI9YgAqAXkMKLXR9TEkFJEW3fR7XTc1wzqw371337FD32EYWo</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Venkatesan, Parthiban</creator><creator>Wu, Shu-Pao</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0002-4424-5486</orcidid></search><sort><creationdate>20150101</creationdate><title>A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application</title><author>Venkatesan, Parthiban ; Wu, Shu-Pao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-476576fe7f781a4d1e0567f9794c0f42badca4e695a61ceb77fc4a309e3094173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Binding</topic><topic>Chemoreceptors</topic><topic>Confocal</topic><topic>Constants</topic><topic>Emission</topic><topic>Fluorescence</topic><topic>Microscopy</topic><topic>Spectral emissivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venkatesan, Parthiban</creatorcontrib><creatorcontrib>Wu, Shu-Pao</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venkatesan, Parthiban</au><au>Wu, Shu-Pao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>53</issue><spage>42591</spage><epage>42596</epage><pages>42591-42596</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>A pyrene-based fluorescent sensor (
PHP
) was synthesized for Cu(
ii
) detection. It had high selectivity towards Cu
2+
ions
via
photoinduced electron transfer (PET) based fluorescence enhancement. In the presence of Cu
2+
,
PHP
provided significant blue emission, while Ag
+
, Al
3+
, Ca
2+
, Cd
2+
, Co
2+
, Cr
3+
, Fe
2+
, Fe
3+
, Hg
2+
, Mg
2+
, Mn
2+
, Ni
2+
, Pb
2+
, and Zn
2+
metal ions produced only minor changes in the fluorescence spectra. The association constant (
K
a
) for Cu
2+
binding to
PHP
had a value of 1.0 × 10
4
M
−1
. The maximum emission change induced by Cu
2+
binding to the chemosensor
PHP
was observed over the pH range 5.0–10.0. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that
PHP
can be used as an effective fluorescent probe for detecting Cu
2+
in living cells.</abstract><doi>10.1039/C5RA05440K</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-4424-5486</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2015-01, Vol.5 (53), p.42591-42596 |
| issn | 2046-2069 2046-2069 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1730098020 |
| source | Royal Society of Chemistry |
| subjects | Binding Chemoreceptors Confocal Constants Emission Fluorescence Microscopy Spectral emissivity |
| title | A turn-on fluorescent pyrene-based chemosensor for Cu( ii ) with live cell application |
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