In vitro evaluation of “horchata” co-products as carbon source for probiotic bacteria growth

• Published in: Food and bioproducts processing 2013-07, Vol.91 (3), p.279-286
• Main Authors: Sánchez-Zapata, Elena, Fernández-López, Juana, Pérez-Alvarez, José A., Soares, José, Sousa, Sergio, Gomes, Ana M.P., Pintado, Manuela M.E.
• Format: Article
• Language: English
• Subjects: Acetic acid; Acidophilus; Bacteria; Bifidobacterium animalis; butyric acid; Carbon; coproducts; culture media; dried skim milk; Glucose; In vitro testing; lactic acid; Lactobacillus; Lactobacillus acidophilus; Liquids; Microdilution assay; Milk; Organic acids; Probiotics; reconstituted milk; screening; Tiger nut
• ISSN: 0960-3085; 1744-3571
• DOI: 10.1016/j.fbp.2012.11.003

[Display omitted] ► Tiger nut co-products can be used as carbon source to promote the bacterial growth. ► Probiotic growing in medium with tiger nut co-products show a faster growth. ► Tiger nut co-products addition increases lactic, acetic and butyric acid production. Tiger nut milk (“horchata”) liquid co-products (TNLC) were evaluated as carbon source for probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis) growth by screening via microplate assay and determination of viable cells and metabolic activity. Based on MRS five different basal media were prepared ((i) without carbon source, (ii) with 2% (w/v) glucose, (iii) with 2% (w/v) FOS, (iv) with 2% (v/v) TNLC and (v) 3% (v/v) TNLC). Additionally, reconstituted TNLC was also used as basal medium. For determination of viable cells and metabolic activity skim milk powder was used. Glucose was found to be the best substrate to L. acidophilus grows, followed by FOS and TNLC (2% and 3%, respectively). TNLC (3%) was found to be the best substrate followed by TNLC (2%), glucose and FOS, in the promotion of growth of B. animalis. The growth of L. acidophilus and B. animalis in skim milk reconstituted TNLC+water was higher than samples inoculated in skim milk reconstituted only with water (p