Helicobacter pylori-associated gastric pro- and antioxidant formation in Mongolian gerbils

• Published in: Free radical biology & medicine 1999-03, Vol.26 (5), p.679-684
• Main Authors: Suzuki, Hidekazu, Mori, Mikiji, Seto, Koichi, Kai, Akemi, Kawaguchi, Chizuko, Suzuki, Masayuki, Suematsu, Makoto, Yoneta, Tomoyuki, Miura, Soichiro, Ishii, Hiromasa
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Antioxidants - metabolism; Free radical; Gastric Mucosa - microbiology; Gastric Mucosa - physiology; Gastric Mucosa - physiopathology; Gerbillinae; Glutathione; Glutathione - metabolism; Glutathione Disulfide - metabolism; Glutathione Peroxidase - metabolism; Helicobacter Infections - physiopathology; Helicobacter pylori; Helicobacter pylori - physiology; Immunoglobulin G - blood; Leukocyte infiltration; Lipid peroxidation; Male; Neutrophils - physiology; Oxidants - metabolism; Peroxidase - metabolism; Superoxide dismutase; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - analysis; Time Factors
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/S0891-5849(98)00248-2

Helicobacter pylori colonized gastric mucosa is manifest in a significant neutrophil infiltration with an extensive level of oxyradical formation. Mongolian gerbil is one of the excellent models for H. pylori-infection. The present study was designed to investigate pro- and antioxidant formation in the stomach of H. pylori-positive gerbils. Fourteen male Mongolian gerbils (MGS/Sea) were orally inoculated with H. pylori (ATCC43504) (Hp group) and 15 gerbils were inoculated with the culture media (Control). H. pylori infection was confirmed by the serum anti- H. pylori IgG test. Each gerbil was evaluated 6 or 12 weeks after the inoculation. Neutrophil infiltration was assessed by the tissue MPO activity. Mucosal oxidative stress was evaluated by thiobarbituric acid-reactive substances (TBARS), total glutathione contents, glutathione peroxidase (GSHPx) activity and Cu-, Zn-superoxide dismutase (SOD) activity. In Hp group, the H. pylori was persistently infected until 12 weeks. The level of MPO activity was significantly higher in Hp group at 6 and 12 weeks. Although the levels of TBARS and total glutathione were within the same range as controls at 6 weeks, they were significantly increased at 12 weeks. However, GSHPx activity was significantly increased at 6 weeks, but became the same range with the controls at 12 weeks. SOD activity showed no significant increase in Hp group at 6 and 12 weeks. In conclusion, H. pylori inoculation induced gastric mucosal neutrophil activation and pro-oxidant formation and also increased total glutathione contents, one of the mucosal antioxidants in gerbils.