rRNA based identification and detection systems for rhizobia and other bacteria

Ribosomal ribonucleic acids are excellent marker molecules for the elucidation of bacterial phylogeny; they also provide useful target sites for identification and detection with nucleic acid probes. Based on the currently available 16S rRNA sequence data, bacteria of the rhizobial phenotype (plant...

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Bibliographic Details
Published in:Plant and soil 1998-07, Vol.204 (1), p.1-19
Main Authors: Ludwig, W. (Technische Univ. Muenchen (Germany). Lehrstuhl fuer Mikrobiologie), Amann, R, Martinez-Romero, E, Schonhuber, W, Bauer, S, Neef, A, Schleifer, K.H
Format: Article
Language:English
Subjects:
ARN
Azorhizobium
Bacteria
Biological taxonomies
Bradyrhizobium
FILOGENIA
FORMATION DE NODOSITES
Heterogeneity
IDENTIFICACION
IDENTIFICATION
Mesorhizobium
Microbiology
Nitrogen fixation
NODULACION
Nucleic acid probes
Nucleic acids
NUCLEOTIDE SEQUENCE
Oligonucleotide probes
Phylogenetics
PHYLOGENIE
PHYLOGENY
Probes
Proteobacteria
RHIZOBIACEAE
Rhizobium
RNA
ROOT NODULATION
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Sinorhizobium
TAXONOMIA
TAXONOMIE
TAXONOMY
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Summary:Ribosomal ribonucleic acids are excellent marker molecules for the elucidation of bacterial phylogeny; they also provide useful target sites for identification and detection with nucleic acid probes. Based on the currently available 16S rRNA sequence data, bacteria of the rhizobial phenotype (plant nodulation, nitrogen fixation) are members of three moderately related phylogenetic sub-groups of the α-subclass of the Proteobacteria: i.e. the rhizobia group, the bradyrhizobia group, and the azorhizobia group. All rhizobia, azo-, brady-, meso- and sinorhizobia are closely related to and in some cases phylogenetically intermixed with, non-symbiotic and/or non-nitrogen-fixing bacteria. Especially in the case of Bradyrhizobium japonicum strains, the 16S rRNA sequence data indicate substantial heterogeneity. Specific probe design and evaluation are discussed. A multiprobe concept for resolving specificity problems with group specific probes is presented. In situ identification with group specific probes of rhizobia in cultures as well as rhizobia and cyanobacteria within plant material is shown.
ISSN:0032-079X
1573-5036
DOI:10.1023/A:1004350708767