Somatic embryogenic tissue establishment from mature Pinus nigra Arn. ssp. Salzmannii embryos

• Published in: In vitro cellular & developmental biology. Plant 1999-05, Vol.35 (3), p.206-209
• Main Authors: Radojevic, L, Alvarez, C, Fraga, M.F, Rodriguez, R
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; application rate; benzyladenine; Biological and medical sciences; Biotechnology; Callus; Conifers; culture media; Developmental biology; Developmental Biology/Morphogenesis; Economic plant physiology; embryo (plant); embryo culture; Embryogenesis; Embryological stage; Embryos; Fundamental and applied biological sciences. Psychology; In vitro culture; Minerals; naphthaleneacetic acid; Pinus nigra; Pinus nigra salzmannii; Plant physiology and development; regenerative ability; Somatic embryogenesis; Somatic embryos; tissue culture; Tissue cultures, protoplasts; Woody plants
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-999-0078-x

Somatic embryogenesis from mature zygotic embryos of$salgare\tilde{n}o$pine (Pinus nigra Arn. ssp. salzmannii) was induced after 2 wk of culture in L1medium [Murashige and Skoog mineral solution with$10.7 \mu M$naphthaleneacetic acid (NAA) and$8.8 \mu M$6-benzyladenine (BA)] or L2medium [Gupta and Durzan mineral solution (DCR)] supplemented with the phytohormone combination as above. Four different combinations of growth regulators,$NAA (2.6-10.7 \mu M)$and$BA (6.6-8.8 \mu M)$, were tried for subsequent passage. The best percentage of embryo induction and manifestation was obtained on DCR medium with$2.6 \mu M NAA$and$6.6-8.8 \mu M BA$. Transfer of isolated somatic embryos from the basal media to L3medium (Quoirin and Le Poivre modified mineral solution without hormones and supplemented with 0.3% activated charcoal), facilitated random embryo maturation and some development into plantlets.