Action potentials and relations to the theta rhythm of medial septal neurons in vivo

• Published in: Experimental brain research 1999-08, Vol.127 (3), p.244-258
• Main Authors: BRAZHNIK, E. S, FOX, S. E
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Anesthesia; Animals; Atropine - pharmacology; Biological and medical sciences; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Extracellular Space - physiology; Fundamental and applied biological sciences. Psychology; GABA Antagonists - pharmacology; Hippocampus - physiology; Intracellular Fluid - physiology; Male; Motor Activity - physiology; Muscarinic Antagonists - pharmacology; Neurons - physiology; Picrotoxin - pharmacology; Rats; Rats, Sprague-Dawley; Septal Nuclei - cytology; Septal Nuclei - physiology; Theta Rhythm; Urethane; Vertebrates: nervous system and sense organs
• ISSN: 0014-4819; 1432-1106
• DOI: 10.1007/s002210050794

The influence of the medial septal nucleus and the nucleus of the diagonal band of Broca (MS-DB) on the hippocampal theta rhythm includes both cholinergic and gamma-aminobutyric acid (GABAergic) components. To understand the intrinsic septal interactions and the separate contributions of the cholinergic and GABAergic septohippocampal neurons to the theta rhythm in behaving animals, it is essential to be able to identify these two classes from extracellular recordings. Here the durations of extracellularly recorded action potentials are compared with the other characteristics of the neurons. Extracellular recordings were taken from neurons of the MS-DB both in freely moving rats (114 cells) and in urethane-anesthetized rats (112 cells). These were compared with intracellular recordings taken from MS-DB neurons in urethane-anesthetized rats (58 cells). Hippocampal EEG was recorded from above the CA1 pyramidal cell layer (CAI theta) and near the hippocampal fissure (dentate theta) to compare the firing phase across cells. Here it is shown that two major types of rhythmically bursting cells in the MS-DB that had been distinguished previously in intracellular recordings in vivo are also separable in extracellular recordings in vivo on the basis of the durations of their action potentials. In both awake and anesthetized rats the main properties of the two cell types were found to differ: firing rate, phase-relation to the hippocampal theta rhythm and sensitivity of their rhythmicity to blockade of muscarinic transmission. As was previously shown for intracellular recordings in anesthetized rats, it is shown here that in awake rats, too, the more rapidly firing brief-spike (putative GABAergic) cells fired with highest probability on the negative phase of the dentate theta, whereas the more slowly firing long-spike (putative cholinergic) cells fired mostly on the positive phase. Previous work showed that in intracellular recordings from anesthetized rats the rhythmic firing of most brief-spike cells was still retained even during muscarinic blockade, but that of most long-spike cells was lost. Here we also report a recategorization according to spike duration of existing extracellular recordings taken from anesthetized rats, confirming the above observation with much larger numbers of cells. Three additional major new findings are also reported here. (1) In awake rats, muscarinic blockade has relatively little effect on either cell type. (2) Under anesthesia, the