ZO-1 expression shows prognostic value in chronic B cell leukemia

• Published in: Immunobiology (1979) 2016-01, Vol.221 (1), p.6-11
• Main Authors: Nesmiyanov, Pavel P, Tolkachev, Boris E, Strygin, Andrey V
• Format: Article
• Language: English
• Subjects: 1-Octanol - pharmacology; ADP-ribosyl Cyclase 1 - genetics; ADP-ribosyl Cyclase 1 - metabolism; Adult; Advanced Basic Science; Aged; Allergy and Immunology; Antibodies - pharmacology; Apoptosis - drug effects; B-cell chronic leukemia; B-Lymphocytes - drug effects; B-Lymphocytes - metabolism; B-Lymphocytes - pathology; Carbenoxolone - pharmacology; Cell Communication - drug effects; Cell Cycle - drug effects; Connexin 43 - antagonists & inhibitors; Connexin 43 - genetics; Connexin 43 - metabolism; Connexin43; Female; Follow-Up Studies; Gap junction intercellular communication; Gene Expression Regulation, Leukemic; Humans; Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis; Leukemia, Lymphocytic, Chronic, B-Cell - genetics; Leukemia, Lymphocytic, Chronic, B-Cell - mortality; Leukemia, Lymphocytic, Chronic, B-Cell - pathology; Male; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Middle Aged; Primary Cell Culture; Prognosis; Signal Transduction; Survival Analysis; ZAP-70 Protein-Tyrosine Kinase - genetics; ZAP-70 Protein-Tyrosine Kinase - metabolism; ZO-1; Zonula Occludens-1 Protein - genetics; Zonula Occludens-1 Protein - metabolism
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/j.imbio.2015.08.008

Abstract Connexin-mediated gap junctions are vital for tumor cell function. Intracellular pathways of connexin signaling use Zonula Occludens protein-1 (ZO-1) as an intermediate. This report describes the ZO-1 and connexin 43 (Cx43) expression pattern in lymphocytes from chronic B-cell leukemia (B-CLL) patients. The ZO-1 and Cx43 expression in the B cells of 113 B-CLL patients was identified. Western blot and flow cytometry were used to determine protein expression. Results indicated that ZO-1 and Cx43 expression was reduced and correlated negatively with CD38 and Zap-70 expression. Inhibition of intercellular communication with anti-Cx43 antibodies, 1-octanol, or carbenoxolone resulted in induced cell apoptosis. These data suggest that ZO-1, along with CD38 and Zap-70, plays a role in cell cycle regulation in B-CLL and may be used as a prognostic marker in B-CLL monitoring.