Spleen function after preservation in a physiological solution

• Published in: The Journal of surgical research 2015-12, Vol.199 (2), p.586-591
• Main Authors: de Matos Filho, Argos Soares, MD, PhD, Petroianu, Andy, MD, PhD
• Format: Article
• Language: English
• Subjects: Animals; Autogenous splenic implant; Damage control; Isotonic Solutions; Lactated Ringer solution; Male; Organ Preservation; Organ Preservation Solutions; Random Allocation; Rats, Sprague-Dawley; Spleen; Spleen - transplantation; Surgery; Trauma
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2015.05.058

Abstract Background The purpose of this study was to evaluate the morphology and function of implanted autogenous spleen tissue after 24 h of preservation in a physiological solution. Material and methods Thirty-five male rats were divided into seven groups ( n  = 5): group 1, without surgical procedure; group 2, total splenectomy; group 3, total splenectomy and immediate implant of autogenous spleen tissue; group 4, total splenectomy and preservation of the entire spleen in lactated Ringer solution at room temperature for 24 h, followed by spleen sectioning and implantation; group 5, total splenectomy, followed by spleen sectioning and preservation in lactated Ringer solution at room temperature for 24 h and subsequent implantation of the slices; group 6, total splenectomy and preservation of the entire spleen in lactated Ringer solution at 4°C for 24 h, followed by spleen sectioning and implantation; and group 7, total splenectomy, the spleen was sliced and preserved in lactate Ringer solution at 4°C for 24 h, followed by implantation of the slices. After 90 d, scintigraphic studies using sulfur colloid labeled with 99mTc of the liver, lungs, spleen, implants, and a blood clot were performed. Hematological (erythrogram, leukogram, and platelets) and histologic studies were carried out. Results The autogenous splenic implants regenerated in all animals that received those implants preserved at 4°C and immediately after excision. The scintigraphic study showed a better phagocytic function in groups 1, 3, 6, and 7. No difference was observed in the hematological study. Conclusions Spleen tissue preserved in lactated Ringer solution at 4°C for 24 h maintains its vitality and capacity to recover hematological and phagocytic functions.