Coenzyme Q10 and α‐Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen

Biotechnology applied for equine semen increases the levels of reactive oxygen species and reduces the natural antioxidant defence, by both dilution and removal of seminal plasma. Therefore, the aims of this study were to evaluate the effect of adding coenzyme Q10 (CoQ10) and α‐tocopherol (α‐TOH) to...

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Bibliographic Details
Published in:Reproduction in domestic animals 2015-12, Vol.50 (6), p.1003-1010
Main Authors: Nogueira, BG, Sampaio, BFB, Souza, MIL, Costa e Silva, EV, Zúccari, CESN
Format: Article
Language:English
Subjects:
adults
alpha-tocopherol
alpha-Tocopherol - pharmacology
Animals
antioxidants
Antioxidants - pharmacology
biotechnology
coenzyme Q10
cooling
ethanol
Horses
lipid peroxidation
Lipid Peroxidation - drug effects
Male
Malondialdehyde - metabolism
reactive oxygen species
Reactive Oxygen Species - metabolism
Semen Preservation - veterinary
seminal plasma
Sperm Motility - drug effects
spermatozoa
stallions
Ubiquinone - analogs & derivatives
Ubiquinone - pharmacology
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Summary:Biotechnology applied for equine semen increases the levels of reactive oxygen species and reduces the natural antioxidant defence, by both dilution and removal of seminal plasma. Therefore, the aims of this study were to evaluate the effect of adding coenzyme Q10 (CoQ10) and α‐tocopherol (α‐TOH) to the cooling extender, singly or in combination, on sperm parameters, and their effectiveness in preventing lipid peroxidation (LPO) of equine semen during cooling at 5°C for 72 h. Ten adult stallions of proven fertility were used, using two ejaculates each, subjecting them to the treatments with the following concentrations: α‐TOH: 2 mm; CoQ10: 40 μg/ml; and CoQ10 + α‐TOH: 40 μg/ml + 2 mm for control (C) without the addition of antioxidants and for vehicle control (EtOH) with 100 μl ethanol. The CoQ10 group had a higher percentage of total motility (69.1 ± 16.2%) compared to control (62.1 ± 16.2%) and EtOH (58.1 ± 18.6%). CoQ10 + α‐TOH and α‐TOH groups were most effective in preventing LPO compared to controls (1765.9 ± 695.9, 1890.8 ± 749.5, 2506.2 ± 769.4 ng malondialdehyde/10⁸ sptz, respectively). In conclusion, CoQ10 and α‐TOH were effective during the cooling process of equine semen at 5°C for 72 h, providing increased levels of total motility, as well as lower LPO.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12627