Human papillomavirus detection in histologic samples of multifocal epithelial hyperplasia: a novel demographic presentation

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2015-12, Vol.120 (6), p.733-743
• Main Authors: Khanal, Sujita, MS, Cole, Eric T., DMD, MS, Joh, Joongho, PhD, Ghim, Shin Je, PhD, Jenson, Alfred Bennett, MD, MS, FACP, Rai, Shesh N., PhD, Trainor, Patrick J., MS, MA, Shumway, Brian S., DDS, MS
• Format: Article
• Language: English
• Subjects: Adult; Biopsy; Dentistry; Female; Focal Epithelial Hyperplasia - virology; Genotype; Humans; Male; Middle Aged; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Polymerase Chain Reaction; Surgery
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2015.07.035

Objective Human papillomavirus (HPV) typing of oral lesions microscopically consistent with multifocal epithelial hyperplasia (MEH) was performed to identify potential novel clinical presentations. Study Design MEH (N = 22 lesions, 17 patients) and squamous papilloma control samples (N = 9 lesions, 9 patients) were compared by using polymerase chain reaction-based HPV genotyping. Student's t tests were used to compare continuous characteristics. Results Of the study cases, 86.4% of MEH and only 11% of controls were positive for HPV ( P  = .0002). In MEH lesions, 45.5% contained HPV32, 36.4% HPV6, and 4.5% HPV40. MEH lesions were mostly multifocal (50%) and occurred in HIV-negative patients (81.3%). They predominated on the labial/buccal mucosa (63.3%), and there were significant differences between groups by anatomic site ( P < .0001). HPV32, but not HPV6, was detected in known HIV-positive patients. Conclusions A novel clinical presentation of MEH associated with HPV32 in HIV-negative, middle-aged to older adults is reported here. One case with HPV40 is the first to be reported. Future detection protocols should include HPV32, as it may be currently overlooked.