CpG-DNA enhances the tight junction integrity of the bronchial epithelial cell barrier

• Published in: Journal of allergy and clinical immunology 2015-11, Vol.136 (5), p.1413-1416.e8
• Main Authors: Kubo, Terufumi, MD, PhD, Wawrzyniak, Paulina, MSc, Morita, Hideaki, MD, PhD, Sugita, Kazunari, MD, PhD, Wanke, Kerstin, PhD, Kast, Jeannette I., MSc, Altunbulakli, Can, MSc, Rückert, Beate, Sci Tec, Jakiela, Bogdan, PhD, Sanak, Marek, MD, PhD, Akdis, Mübeccel, MD, PhD, Akdis, Cezmi A., MD
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Asthma; Asthma - immunology; Bronchi - cytology; Cell Membrane Permeability - drug effects; Cells, Cultured; Claudin-4 - genetics; Claudin-4 - metabolism; Cytokines; Deoxyribonucleic acid; DNA; Electric Impedance; Epithelial Cells - drug effects; Epithelial Cells - physiology; Gene expression; Humans; Hygiene Hypothesis; Hypotheses; Inflammation; Interleukin-13 - metabolism; Investigations; Ligands; Oligodeoxyribonucleotides - pharmacology; Permeability; Software; Tight Junctions - drug effects; Tight Junctions - genetics; Toll-Like Receptor 9 - agonists; Up-Regulation; Zonula Occludens-1 Protein - genetics; Zonula Occludens-1 Protein - metabolism
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2015.05.006

[...]bronchial epithelial cells are at the forefront of tissue defense and the innate immune response, preventing invasion of tissues as a physical barrier. CpG-2006 stimulation remarkably restored the impaired TJ immunostaining of bronchial epithelial ALI cultures from asthmatic patients (Fig 2, B, and see Fig E5, B), which is consistent with the TER and FITC-dextran flux measurements shown above. [...]improvement of the barrier in bronchial epithelial cells by CpG-2006 seems to be mediated by enhanced expression of TJ-related molecules and their appropriate distribution on cell-cell borders.