Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae

The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase preparation free of an exoinulinase and an extracellular invertase in the culture medium. A recombinant yea...

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Bibliographic Details
Published in:Biotechnology letters 1999-07, Vol.21 (7), p.621-623
Main Authors: Kim, H.S, Lee, D.W, Ryu, E.J, Uhm, T.B, Yang, M.S, Kim, J.B, Chae, K.S
Format: Article
Language:English
Subjects:
Aspergillus ficuum
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
gene expression
Genetic engineering
Genetic technics
Kluyveromyces marxianus
Methods. Procedures. Technologies
Modification of gene expression level
Saccharomyces cerevisiae
Yeast
Yeasts
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Summary:The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase preparation free of an exoinulinase and an extracellular invertase in the culture medium. A recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony, when inulin was included in the medium.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005567403830