Transactivation Activity of Human, Zebrafish, and Rainbow Trout Aryl Hydrocarbon Receptors Expressed in COS-7 Cells: Greater Insight into Species Differences in Toxic Potency of Polychlorinated Dibenzo-p-dioxin, Dibenzofuran, and Biphenyl Congeners

• Published in: Toxicology and applied pharmacology 1999-08, Vol.159 (1), p.41-51
• Main Authors: Abnet, Christian C., Tanguay, Robert L., Heideman, Warren, Peterson, Richard E.
• Format: Article
• Language: English
• Subjects: AhR agonist; Animals; Benzofurans - toxicity; Biological and medical sciences; COS Cells - drug effects; Danio rerio; dioxin; Dose-Response Relationship, Drug; Environmental Pollutants - toxicity; Environmental pollutants toxicology; Fishes; General aspects; Humans; Medical sciences; Oncorhynchus mykiss; PCDDs; Polychlorinated Biphenyls - toxicity; Polychlorinated Dibenzodioxins - analogs & derivatives; Polychlorinated Dibenzodioxins - toxicity; rainbow trout; Receptors, Aryl Hydrocarbon - drug effects; Soil Pollutants - toxicity; Species Specificity; TCDD; Toxicology; Transcriptional Activation - genetics; Transcriptional Activation - physiology; Zebrafish
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1006/taap.1999.8719

Transactivation assays were used to compare the potency and efficacy of polychlorinated dibenzo-p-dioxin (PCDD), dibenzofuran (PCDF), and biphenyl (PCB) congeners in activating aryl hydrocarbon receptors (AhRs) from rainbow trout (rtAhR2α and rtAhR2β), zebrafish (zfAhR2), and human (huAhR), respectively. All AhRs were expressed with their species-specific AhR nuclear translocator (ARNT) in COS-7 cells. Transactivation activity was determined for two PCDD, two PCDF, and seven PCB congeners with each of the four AhR/ARNT pairs using prt1Aluc, a luciferase reporter driven by two dioxin-responsive enhancer elements (DREs) from the rainbow trout cyp1A gene. Maximal-fold induction, EC50, and relative potency values were calculated for congeners that exhibited dose-related activity in the assay. Of the four AhR/ARNT pairs tested with PCDD, PCDF, and non-ortho PCB congeners, three exhibited high activity (rainbow trout AhR2α, zebrafish AhR2, and human AhR), while rainbow trout AhR2β had very weak or no activity. Comparisons between these AhRs showed that while mono-ortho PCBs were able to activate the human AhR, they were generally ineffective in activating rainbow trout and zebrafish AhR2s. This supports the hypothesis that structural differences between mammalian and fish AhRs may account for differences in relative potencies of the mono-ortho PCBs between mammals and fish. Another important finding was a significant difference in transactivation activity between the two rainbow trout AhR2 isoforms despite the fact that they are 95% identical at the amino acid level. For all PCDD, PCDF, and PCB agonists tested, rainbow trout AhR2α was significantly more active than AhR2β. However, rainbow trout AhR2β is active as a 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-activated transcription factor, with enhancer elements from the mouse cyp1A gene. This suggests that AhR2β may have evolved to serve a different physiological function than AhR2α in salmonid fish species.