Natural Occurrence of Wild tomato mosaic virus in Wild Eggplant in China

In winter 2013, typical symptoms of green crinkling and mosaic were observed on wild eggplant leaves in field in Hainan, China. The causal pathogen was identified to be a Wild tomato mosaic virus (WTMV) based upon reverse transcription‐touchdown PCR with degenerate primers. A specific product of 170...

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Bibliographic Details
Published in:Journal of phytopathology 2015-12, Vol.163 (11-12), p.1023-1026
Main Authors: Zhang, Shaoyan, Yu, Naitong, Wang, Xiang, Liang, Jie, Xie, Huimin, Wang, Jianhua, Zhang, Yuliang, Liu, Zhixin
Format: Article
Language:English
Subjects:
amino acids
eggplants
leaves
nucleotide sequences
pathogens
phylogeny
polymerase chain reaction
polypeptides
reverse transcription
Tomato mosaic virus
touchdown PCR
wild eggplant
Wild tomato mosaic virus
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Summary:In winter 2013, typical symptoms of green crinkling and mosaic were observed on wild eggplant leaves in field in Hainan, China. The causal pathogen was identified to be a Wild tomato mosaic virus (WTMV) based upon reverse transcription‐touchdown PCR with degenerate primers. A specific product of 1700 bp containing the partial sequence of the NIb (~950 bp) and CP (~750 bp) coding regions of WTMV was amplified, and the predicted polypeptide consisted of 572 amino acids (Mr 65.52 kDa). This isolate was subsequently named WTMV‐Hainan (GenBank accession KF918754) isolate, and it shared sequence identities of 91% and 95% with WTMV‐Laichau isolate at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the CP gene sequences provided further evidence that all WTMV isolates formed a high‐confidence subclade and WTMV‐Hainan most closely related to WTMV‐KAN, WTMV‐Laichau and WTMV‐GD1. To our knowledge, this is the first report of the natural infection of WTMV on wild eggplant in China.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.12330