Low dose EGCG treatment beginning in adolescence does not improve cognitive impairment in a Down syndrome mouse model

• Published in: Pharmacology, biochemistry and behavior biochemistry and behavior, 2015-11, Vol.138, p.70-79
• Main Authors: Stringer, Megan, Abeysekera, Irushi, Dria, Karl J., Roper, Randall J., Goodlett, Charles R.
• Format: Article
• Language: English
• Subjects: Aging - psychology; Animals; Behavior, Animal - drug effects; Brain Chemistry - drug effects; Catechin - administration & dosage; Catechin - analogs & derivatives; Catechin - therapeutic use; Cognition; Cognition Disorders - drug therapy; Cognition Disorders - etiology; Cognition Disorders - psychology; Down syndrome; Down Syndrome - complications; Down Syndrome - psychology; Dyrk Kinases; Female; Growth - drug effects; Learning - drug effects; Maze Learning - drug effects; Mice; Mice, Transgenic; Mouse model; Protein Serine-Threonine Kinases - antagonists & inhibitors; Protein Serine-Threonine Kinases - metabolism; Protein-Tyrosine Kinases - antagonists & inhibitors; Protein-Tyrosine Kinases - metabolism; Recognition, Psychology - drug effects; Treatment; Trisomy 21
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/j.pbb.2015.09.002

Down syndrome (DS) or Trisomy 21 causes intellectual disabilities in humans and the Ts65Dn DS mouse model is deficient in learning and memory tasks. DYRK1A is triplicated in DS and Ts65Dn mice. Ts65Dn mice were given up to ~20mg/kg/day epigallocatechin-3-gallate (EGCG), a Dyrk1a inhibitor, or water beginning on postnatal day 24 and continuing for three or seven weeks, and were tested on a series of behavioral and learning tasks, including a novel balance beam test. Ts65Dn as compared to control mice exhibited higher locomotor activity, impaired novel object recognition, impaired balance beam and decreased spatial learning and memory. Neither EGCG treatment improved performance of the Ts65Dn mice on these tasks. Ts65Dn mice had a non-significant increase in Dyrk1a activity in the hippocampus and cerebellum. Given the translational value of the Ts65Dn mouse model, further studies will be needed to identify the EGCG doses (and mechanisms) that may improve cognitive function. •Low dose treatment of epigallocatechin-3-gallate did not rescue cognitive deficits.•A novel motor coordination task revealed trisomic deficits in a balance beam task.•EGCG treatment of Ts65Dn mice during adolescence