Molecular cloning and characterization of disease-resistance gene candidates of the nucleotide binding site (NBS) type from Cocos nucifera L

In this study, degenerate primers were used to amplify nucleotide-binding site (NBS)-type sequences from coconut ecotypes either resistant or susceptible to lethal yellowing. Genomic DNA fragments of approximately 500–700 bp were obtained and sequenced. Phylogenetic analysis of these fragments revea...

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Published in:Physiological and molecular plant pathology 2015-01, Vol.89, p.87-96
Main Authors: Puch-Hau, Carlos, Oropeza-Salín, Carlos, Peraza-Echeverría, Santy, Gongora-Paredes, Manuel, Córdova-Lara, Ivan, Narvaez-Cab, María, Zizumbo-Villareal, Daniel, Sáenz-Carbonell, Luis
Format: Article
Language:English
Subjects:
Coconut palm
Cocos nucifera
Lethal yellowing
R genes
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Summary:In this study, degenerate primers were used to amplify nucleotide-binding site (NBS)-type sequences from coconut ecotypes either resistant or susceptible to lethal yellowing. Genomic DNA fragments of approximately 500–700 bp were obtained and sequenced. Phylogenetic analysis of these fragments revealed that they clustered in seven different clades. We also found that all CnRGC sequences were grouped within the non-TIR-NBS-LRR subclass of NBS-LRR genes. The expression analysis revealed changes in expression profiles in response to salicylic acid (SA) and a constitutive expression profile in plants untreated with SA. This is the first large scale analysis of NBS-type sequences in coconut palm. •We found 143 sequences that presented high homology to the NBS-LRR class of R genes.•Phylogenetic analysis revealed that they clustered in seven different clades.•We found that all sequences were grouped within the non-TIR-NBS-LRR subclass.•The expression analysis revealed changes in expression profiles in response to SA.•This is the first large scale analysis of NBS-type sequences in coconut palm.
ISSN:0885-5765
1096-1178
DOI:10.1016/j.pmpp.2015.01.002