Greater filamin C, GSK3[alpha], and GSK3[beta] serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats

Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-spe...

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Bibliographic Details
Published in:Mechanisms of ageing and development 2013-02, Vol.134 (1-2), p.60-63
Main Authors: Sequea, Donel A, Sharma, Naveen, Arias, Edward B, Cartee, Gregory D
Format: Article
Language:English
Online Access:Get full text
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Summary:Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-specific phosphorylation of five Akt substrates (AS160Ser588, TBC1D1Thr596, FLNcSer2213, GSK3[alpha]Ser21, and GSK3[beta]Ser9) in predominantly fast-twitch (epitrochlearis) and predominantly slow-twitch (soleus) muscles. We observed no CR-effect on phosphorylation of AS160Ser588 or TBC1D1Thr596, but there was a CR-induced increase in insulin-stimulated FLNcSer2213, GSK3[alpha]Ser21, and GSK3[beta]Ser9 phosphorylation for both muscles. These results indicate that CR does not uniformly affect insulin-mediated phosphorylation of Akt substrates in fast- or slow-twitch muscles from 24 month-old rats.
ISSN:0047-6374