Biochanin A protects dopaminergic neurons against lipopolysaccharide-induced damage and oxidative stress in a rat model of Parkinson's disease

• Published in: Pharmacology, biochemistry and behavior biochemistry and behavior, 2015-11, Vol.138, p.96-103
• Main Authors: Wang, Jun, He, Can, Wu, Wang-Yang, Chen, Feng, Wu, Yang-Yang, Li, Wei-Zu, Chen, Han-Qing, Yin, Yan-Yan
• Format: Article
• Language: English
• Subjects: Animals; Behavior, Animal - drug effects; Biochanin A; Cicer arietinum; Dopaminergic neuron; Dopaminergic Neurons - drug effects; Enzyme Activation - drug effects; Genistein - pharmacology; Glutathione Peroxidase - biosynthesis; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - toxicity; Macrophage Activation - drug effects; Male; Malondialdehyde - metabolism; Microglia; Microglia - drug effects; NADPH oxidase; NADPH Oxidases - metabolism; Neuroprotective Agents - pharmacology; Oxidative stress; Oxidative Stress - drug effects; Parkinson Disease, Secondary - metabolism; Parkinson Disease, Secondary - pathology; Parkinson Disease, Secondary - prevention & control; Parkinson's disease; Rats; Rats, Sprague-Dawley; Superoxide Dismutase - biosynthesis
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/j.pbb.2015.09.013

Parkinson's disease (PD) is the second most common neurodegenerative disease, which is characterized by loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Accumulated evidences have suggested that oxidative stress is closely associated with the dopaminergic neurodegeneration of PD that can be protected by antioxidants. Biochanin A that is an O-methylated isoflavone in chickpea is investigated to explore its protective mechanism on dopaminergic neurons of the unilateral lipopolysaccharide (LPS)-injected rat. The results showed that biochanin A significantly improved the animal model's behavioral symptoms, prevented the loss of dopaminergic neurons and inhibited the deleterious microglia activation in the LPS-induced rats. Moreover, biochanin A inhibited nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) activation and malondialdehyde (MDA) production, increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in the rat brain. These results suggested that biochanin A might be a natural candidate with protective properties on dopaminergic neurons against the PD. •Biochanin A could alleviate the animal's lesion induced by LPS and apomorphine.•Biochanin A treated increased SOD and GPx activities and the decreased MDA content in LPS rats.•Biochanin A inhibited LPS-induced microglial activation and protected dopaminergic neurodegeneration in the SNpc.•Biochanin A inhibited the expression of p47phox, p67phox and gp91phox in the SNpc.