Disturbances to neurotransmitter levels and their metabolic enzyme activity in a freshwater planarian exposed to cadmium

• Published in: Neurotoxicology (Park Forest South) 2015-03, Vol.47, p.72-81
• Main Authors: Wu, Jui-Pin, Li, Mei-Hui, Chen, Jhih-Sheng, Chung, Szu-Yao, Lee, Hui-Ling
• Format: Article
• Language: English
• Subjects: 5-Hydroxyindole-3-acetic acid (5-HIAA); Animals; Cadmium - pharmacology; Catechol O-Methyltransferase - metabolism; Dopamine - metabolism; Dugesia japonica; Invertebrates; Metal; Monoamine Oxidase - metabolism; Neurotoxicity; Neurotransmitter Agents - metabolism; Planarians - drug effects; Planarians - enzymology; Planarians - metabolism; Serotonin - metabolism
• ISSN: 0161-813X; 1872-9711
• DOI: 10.1016/j.neuro.2015.01.003

•We demonstrated the occurrence of 5-hydroxyindole-3-acetic acid in a planarian.•The substrate- and inhibitor-specificity of planarian MAO activity was observed.•Catechol-O-methyl transferase activity in the planarian was undetectable.•Altered dopamine and serotonin levels were observed in Cd-treated planarians.•Altered MAO-A and -B activities were also observed in Cd-treated planarians. Using specific neurobehaviors as endpoints, previous studies suggested that planarian neurotransmission systems could be targets of Cd neurotoxicity. However, direct evidence for disturbed neurotransmission systems by Cd in treated planarians is still lacking. In planarians, dopamine (DA) and serotonin (5-HT) play critical roles in neuromuscular function, but little is known about their metabolic degradation. Therefore, in this study, we attempted to determine the appearances of DA, 5-HT, and their metabolic products in the freshwater planarian Dugesia japonica, characterize the activity of enzymes involved in their metabolism, and investigate the effects of Cd on planarian 5-HTergic and DAergic neurotransmission systems. Only DA, 5-HT, and 5-hydroxyindole-3-acetic acid (5-HIAA) were found in planarian tissues. Further enzymatic study revealed the activity of planarian monoamine oxidase (MAO) but not catechol-O-methyl transferase (COMT). These findings suggest that planarian MAO catalyzes the metabolism of 5-HT into 5-HIAA. However, DA metabolites from the MAO-involved metabolic pathway were not found, which might be due to a lack of COMT activity. Finally, in Cd-treated planarians, tissue levels of 5-HT and DA were decreased and MAO activity altered, suggesting that planarian neurotransmission systems are disturbed following Cd treatment.