Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella)

The role of leucine (Leu) in the regulation of the intestinal immune status, immune-related signalling molecules and tight junction (TJ) protein transcript abundance in the intestine of grass carp (Ctenopharyngodon idella) was investigated. Six iso-nitrogenous diets that contained graded levels of L...

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Published in:Aquaculture 2015-07, Vol.444, p.134-142
Main Authors: Jiang, Wei-Dan, Deng, Yu-Ping, Liu, Yang, Qu, Biao, Jiang, Jun, Kuang, Sheng-Yao, Tang, Ling, Tang, Wu-Neng, Wu, Pei, Zhang, Yong-An, Zhou, Xiao-Qiu, Feng, Lin
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Language:English
Subjects:
Amino acids
Carp
Ctenopharyngodon idella
Dietary supplements
Freshwater
Intestinal immune
Leucine
Nuclear factor κB p65
Signal transduction
Target of rapamycin
Tight junction
Transcription factors
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cited_by cdi_FETCH-LOGICAL-c382t-3e464eb5df84c6075d9fe24491d4220726b3f778eff60910f492667b1003bc303
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container_title Aquaculture
container_volume 444
creator Jiang, Wei-Dan
Deng, Yu-Ping
Liu, Yang
Qu, Biao
Jiang, Jun
Kuang, Sheng-Yao
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Tang, Wu-Neng
Wu, Pei
Zhang, Yong-An
Zhou, Xiao-Qiu
Feng, Lin
description The role of leucine (Leu) in the regulation of the intestinal immune status, immune-related signalling molecules and tight junction (TJ) protein transcript abundance in the intestine of grass carp (Ctenopharyngodon idella) was investigated. Six iso-nitrogenous diets that contained graded levels of Leu (7.1–17.1gLeukg−1 diets) were fed to the fish for 8weeks. Compared with the control group, appropriate Leu supplementation increased (P
doi_str_mv 10.1016/j.aquaculture.2015.04.005
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Six iso-nitrogenous diets that contained graded levels of Leu (7.1–17.1gLeukg−1 diets) were fed to the fish for 8weeks. Compared with the control group, appropriate Leu supplementation increased (P&lt;0.05) the following: (1) the lysozyme activity, acid phosphatase activity and complement component 3 (C3) content in all intestinal segments; (2) the mRNA levels of interleukin 10, inhibitor factor κBα (IκBα) and target of rapamycin (TOR) in the mid and distal intestine as well as transforming growth factor β1 in all intestinal segments; and (3) the transcript levels for claudin b, claudin 3, claudin 15, occludin and zonula occludens-1 (ZO-1) in the intestine of young grass carp. At the same time, appropriate Leu supplementation down-regulated the mRNA levels of tumour necrosis factor α, interleukin 8 and nuclear factor κB p65 (NF-κB p65) in the mid and distal intestine of young grass carp (P&lt;0.05). Interestingly, the transcript levels for claudin c and claudin 12 showed no significant differences among the groups in the intestine of young grass carp. In conclusion, the positive effect of Leu on intestinal health is associated with the improvement of the intestinal immune status and the regulation of immune-related signalling molecules and tight junction transcripts of fish. •Leu regulated cytokines in three intestinal segments differently.•Leu regulated intestinal NF-κB p65, IκBα and TOR mRNA levels of fish.•Leu regulated intestinal tight junction protein transcript abundance.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2015.04.005</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino acids ; Carp ; Ctenopharyngodon idella ; Dietary supplements ; Freshwater ; Intestinal immune ; Leucine ; Nuclear factor κB p65 ; Signal transduction ; Target of rapamycin ; Tight junction ; Transcription factors</subject><ispartof>Aquaculture, 2015-07, Vol.444, p.134-142</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright Elsevier Sequoia S.A. 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Six iso-nitrogenous diets that contained graded levels of Leu (7.1–17.1gLeukg−1 diets) were fed to the fish for 8weeks. Compared with the control group, appropriate Leu supplementation increased (P&lt;0.05) the following: (1) the lysozyme activity, acid phosphatase activity and complement component 3 (C3) content in all intestinal segments; (2) the mRNA levels of interleukin 10, inhibitor factor κBα (IκBα) and target of rapamycin (TOR) in the mid and distal intestine as well as transforming growth factor β1 in all intestinal segments; and (3) the transcript levels for claudin b, claudin 3, claudin 15, occludin and zonula occludens-1 (ZO-1) in the intestine of young grass carp. At the same time, appropriate Leu supplementation down-regulated the mRNA levels of tumour necrosis factor α, interleukin 8 and nuclear factor κB p65 (NF-κB p65) in the mid and distal intestine of young grass carp (P&lt;0.05). 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Six iso-nitrogenous diets that contained graded levels of Leu (7.1–17.1gLeukg−1 diets) were fed to the fish for 8weeks. Compared with the control group, appropriate Leu supplementation increased (P&lt;0.05) the following: (1) the lysozyme activity, acid phosphatase activity and complement component 3 (C3) content in all intestinal segments; (2) the mRNA levels of interleukin 10, inhibitor factor κBα (IκBα) and target of rapamycin (TOR) in the mid and distal intestine as well as transforming growth factor β1 in all intestinal segments; and (3) the transcript levels for claudin b, claudin 3, claudin 15, occludin and zonula occludens-1 (ZO-1) in the intestine of young grass carp. At the same time, appropriate Leu supplementation down-regulated the mRNA levels of tumour necrosis factor α, interleukin 8 and nuclear factor κB p65 (NF-κB p65) in the mid and distal intestine of young grass carp (P&lt;0.05). 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subjects Amino acids
Carp
Ctenopharyngodon idella
Dietary supplements
Freshwater
Intestinal immune
Leucine
Nuclear factor κB p65
Signal transduction
Target of rapamycin
Tight junction
Transcription factors
title Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella)
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