Purification of dendritic cell and macrophage subsets from the normal mouse small intestine

Mononuclear phagocytes are essential for protecting against pathogens breaching the intestinal mucosa and maintaining the integrity of the gastrointestinal tract. The mononuclear phagocyte family of the healthy intestine is represented by a small population of hematopoietic cells including dendritic...

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Bibliographic Details
Published in:Journal of immunological methods 2015-06, Vol.421, p.1-13
Main Authors: Koscsó, Balázs, Gowda, Kavitha, Schell, Todd D., Bogunovic, Milena
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Basic-Leucine Zipper Transcription Factors - biosynthesis
Basic-Leucine Zipper Transcription Factors - genetics
Dendritic cells
Dendritic Cells - classification
Dendritic Cells - cytology
Female
Flow cytometry
Flow Cytometry - methods
fms-Like Tyrosine Kinase 3 - biosynthesis
fms-Like Tyrosine Kinase 3 - genetics
Gene Expression Profiling
Gut
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Intestine, Small - cytology
Intestine, Small - immunology
Macrophages
Macrophages - classification
Macrophages - cytology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mononuclear phagocytes
Repressor Proteins - biosynthesis
Repressor Proteins - genetics
Sequence Analysis, DNA
Small intestine
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Summary:Mononuclear phagocytes are essential for protecting against pathogens breaching the intestinal mucosa and maintaining the integrity of the gastrointestinal tract. The mononuclear phagocyte family of the healthy intestine is represented by a small population of hematopoietic cells including dendritic cells and macrophages. Distinct mononuclear phagocyte subsets strategically accumulate within and below the mucosal epithelium and are distributed in the submucosa and muscularis externa. Shaped by its unique microenvironment, each mononuclear phagocyte subset is developmentally and functionally unique and phenotypically distinct. Here we summarize our recent advances on identifying and purifying various intestinal mononuclear phagocyte subsets by flow cytometry in the context of their developmental properties and location within the intestinal tissue.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2015.02.013