Fibroblast growth factor 21 protects mouse brain against d-galactose induced aging via suppression of oxidative stress response and advanced glycation end products formation

• Published in: Pharmacology, biochemistry and behavior biochemistry and behavior, 2015-06, Vol.133, p.122-131
• Main Authors: Yu, Yinhang, Bai, Fuliang, Wang, Wenfei, Liu, Yaonan, Yuan, Qingyan, Qu, Susu, Zhang, Tong, Tian, Guiyou, Li, Siming, Li, Deshan, Ren, Guiping
• Format: Article
• Language: English
• Subjects: AGEs; Aging; Aging - drug effects; Animals; Brain - drug effects; Brain - metabolism; Cognition Disorders - chemically induced; Cognition Disorders - drug therapy; d-galactose; FGF21; Fibroblast Growth Factors - pharmacology; Fibroblast Growth Factors - therapeutic use; Galactose - pharmacology; Glycation End Products, Advanced - drug effects; Inflammation; Inflammation Mediators - metabolism; Male; Mice; Oxidative stress; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/j.pbb.2015.03.020

Fibroblast growth factor 21 (FGF21) is a hormone secreted predominantly in the liver, pancreas and adipose tissue. Recently, it has been reported that FGF21-Transgenic mice can extend their lifespan compared with wild type counterparts. Thus, we hypothesize that FGF21 may play some roles in aging of organisms. In this study d-galactose (d-gal)-induced aging mice were used to study the mechanism that FGF21 protects mice from aging. The three-month-old Kunming mice were subcutaneously injected with d-gal (180mg·kg−1·d−1) for 8weeks and administered simultaneously with FGF21 (1, 2 or 5mg·kg−1·d−1). Our results showed that administration of FGF21 significantly improved behavioral performance of d-gal-treated mice in water maze task and step-down test, reduced brain cell damage in the hippocampus, and attenuated the d-gal-induced production of MDA, ROS and advanced glycation end products (AGEs). At the same time, FGF21 also markedly renewed the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total anti-oxidation capability (T-AOC), and decreased the enhanced total cholinesterase (TChE) activity in the brain of d-gal-treated mice. The expression of aldose reductase (AR), sorbitol dehydrogenase (SDH) and member-anchored receptor for AGEs (RAGE) declined significantly after FGF21 treatment. Furthermore, FGF21 suppressed inflamm-aging by inhibiting IκBα degradation and NF-κB p65 nuclear translocation. The expression levels of pro-inflammatory cytokines, such as TNF-α and IL-6, decreased significantly. In conclusion, these results suggest that FGF21 protects the aging mice brain from d-gal-induced injury by attenuating oxidative stress damage and decreasing AGE formation. •FGF21 could improve behavioral performance of aging mice induced by d-gal.•FGF21 could reduce brain cell damage in the hippocampus of aging mice.•FGF21 prevents aging mainly via suppression of oxidative stress response.•FGF21 prevents aging through inhibiting AGE production.•FGF21 suppressed inflamm-aging by inhibiting NF-κB p65 nuclear translocation.