It's a dirty job — A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material

We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical s...

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Bibliographic Details
Published in:Journal of microbiological methods 2015-06, Vol.113, p.10-12
Main Authors: Andersson, Sofia, Sikora, Per, Karlberg, Maria L., Winiecka-Krusnell, Jadwiga, Alm, Erik, Beser, Jessica, Arrighi, Romanico B.G.
Format: Article
Language:English
Subjects:
Clinical parasitology
Cryptosporidiosis - parasitology
Cryptosporidium
Cryptosporidium - genetics
Cryptosporidium - isolation & purification
DNA, Protozoan - analysis
Feces - parasitology
Genome sequencing
Genome, Protozoan
Humans
Immunomagnetic Separation
Oocysts
Polymerase Chain Reaction
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Description
Summary:We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical specimens.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2015.03.018