Modified expression of peripheral blood lymphocyte muscarinic cholinergic receptors in asthmatic children

Abstract Lymphocytes possess an independent cholinergic system. We assessed the expression of muscarinic cholinergic receptors in lymphocytes from 49 asthmatic children and 10 age matched controls using Western blot. We demonstrated that CD4 + and CD8 + T cells expressed M2 and M4 muscarinic recepto...

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Bibliographic Details
Published in:Journal of neuroimmunology 2015-07, Vol.284, p.37-43
Main Authors: Cherubini, Emanuela, Tabbì, Luca, Scozzi, Davide, Mariotta, Salvatore, Galli, Elena, Carello, Rossella, Avitabile, Simona, Tayebati, Seyed Koshrow, Amenta, Francesco, De Vitis, Claudia, Mancini, Rita, Ricci, Alberto
Format: Article
Language:English
Subjects:
Administration, Inhalation
Adult
Age Factors
Allergy and Immunology
Asthma
Asthma - blood
Asthma - diagnosis
Asthma - metabolism
Asthma - pathology
Bronchodilator Agents - administration & dosage
Case-Control Studies
CD4 Antigens - metabolism
CD8 Antigens - metabolism
Child
Female
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
Human
Humans
Linear Models
Lymphocytes - drug effects
Lymphocytes - metabolism
Male
Muscarinic receptors
Neurology
Nitric Oxide - administration & dosage
Receptors, Muscarinic - genetics
Receptors, Muscarinic - metabolism
Severity of Illness Index
Statistics, Nonparametric
Western blot
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Summary:Abstract Lymphocytes possess an independent cholinergic system. We assessed the expression of muscarinic cholinergic receptors in lymphocytes from 49 asthmatic children and 10 age matched controls using Western blot. We demonstrated that CD4 + and CD8 + T cells expressed M2 and M4 muscarinic receptors which density were significantly increased in asthmatic children in comparison with controls. M2 and M4 receptor increase was strictly related with IgE and fraction of exhaled nitric oxide (FeNO) measurements and with impairment in objective measurements of airway obstruction. Increased lymphocyte muscarinic cholinergic receptor expression may concur with lung cholinergic dysfunction and with inflammatory molecular framework in asthma.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2015.04.016