Brevibacterium metallicus sp. nov., an endophytic bacterium isolated from roots of Prosopis laegivata grown at the edge of a mine tailing in Mexico

• Published in: Archives of microbiology 2015-12, Vol.197 (10), p.1151-1158
• Main Authors: Román-Ponce, Brenda, Li, Yong Hua, Vásquez-Murrieta, María Soledad, Sui, Xin Hua, Chen, Wen Feng, Estrada-de Los Santos, Paulina, Wang, En Tao
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Typing Techniques; Base Composition - genetics; Brevibacterium; Brevibacterium - classification; Brevibacterium - genetics; Brevibacterium - isolation & purification; Brevibacterium - metabolism; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty Acids - analysis; Gram-positive bacteria; Heavy metals; Metals, Heavy - metabolism; Mexico; Mine tailings; Mine wastes; Mining; Nucleic Acid Hybridization; Phylogenetics; Phylogeny; Plant Roots - microbiology; Plant tissues; Prosopis; Prosopis - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Symbiosis - physiology; Taxonomy; Vitamin K 2 - analysis
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-015-1156-6

A Gram-positive, aerobic, nonmotile strain, NM2E3(T) was identified as Brevibacterium based on the 16S rRNA gene sequence analysis and had the highest similarities to Brevibacterium jeotgali SJ5-8(T) (97.3 %). This novel bacterium was isolated from root tissue of Prosopis laegivata grown at the edge of a mine tailing in San Luis Potosí, Mexico. Its cells were non-spore-forming rods, showing catalase and oxidase activities and were able to grow in LB medium added with 40 mM Cu(2+), 72 mM As(5+) and various other toxic elements. Anteiso-C15:0 (41.6 %), anteiso-C17:0 (30 %) and iso-C15:0 (9.5 %) were the major fatty acids. MK-8(H2) (88.4 %) and MK-7(H2) (11.6 %) were the major menaquinones. The DNA G + C content of the strain NM2E3(T) was 70.8 mol % (Tm). DNA-DNA hybridization showed that the strain NM2E3(T) had 39.8, 21.7 and 20.3 % relatedness with B. yomogidense JCM 17779(T), B. jeotgali JCM 18571(T) and B. salitolerans TRM 45(T), respectively. Based on the phenotypic and genotypic analyses, the strain NM2E3(T) (=CCBAU 101093(T) = HAMBI 3627(T) = LMG 8673(T)) is reported as a novel species of the genus Brevibacterium, for which the name Brevibacterium metallicus sp. nov., is proposed.