Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059

• Published in: Journal of industrial microbiology & biotechnology 2015-12, Vol.42 (12), p.1609-1621
• Main Authors: Białkowska, Aneta M, Gromek, Ewa, Krysiak, Joanna, Sikora, Barbara, Kalinowska, Halina, Jędrzejczak-Krzepkowska, Marzena, Kubik, Celina, Lang, Siegmund, Schütt, Fokko, Turkiewicz, Marianna
• Format: Article
• Language: English
• Subjects: apple pomace; Apples; Aspergillus niger - enzymology; Bacillus - metabolism; Bacillus licheniformis; Bacteria; Batch Cell Culture Techniques; batch fermentation; Batch processes; Biochemistry; Bioinformatics; Biomedical and Life Sciences; Bioreactors - microbiology; Biotechnology; Butylene Glycols - metabolism; By products; Cell Culture and Bioengineering; Cellulose; containers; Dietary minerals; Enzymes; Fermentation; fermenters; Food; Fruit juices; Genetic Engineering; Glucose; Glucose - metabolism; hydrolysates; Hydrolysis; Inorganic Chemistry; Life Sciences; Lignin; Lignocellulose; Malus - chemistry; Malus - metabolism; Manufacturing; Microbiology; minerals; Raw materials; Studies
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-015-1697-3

2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113 g/L (in 163 h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27 g/L (in 32 h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71 g/L 2,3-BD in 160 h, and 72.39 g/L 2,3-BD in 94 h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals.