Fermentation optimization of a Saccharomyces cerevisiae strain producing 1F9 recombinant spidroin

• Published in: Applied biochemistry and microbiology 2015-12, Vol.51 (7), p.766-773
• Main Authors: Sidoruk, K. V, Davydova, L. I, Kozlov, D. G, Gubaidullin, D. G, Glazunov, A. V, Bogush, V. G, Debabov, V. G
• Format: Article
• Language: English
• Subjects: Biochemistry; biomass; Biomedical and Life Sciences; casein hydrolysates; culture media; Fermentation; fructose; galactose; glucose; Life Sciences; Medical Microbiology; Microbiology; Optimization techniques; Peptones; Saccharomyces cerevisiae; sucrose; Technology of Biopreparations; tryptones; Yeast; yeast extract; Yeasts
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683815070066

The fermentation of a Saccharomyces cerevisiae strain producing recombinant spidroin IF9 was optimized. A simplified two-stage scheme of the process was developed; the effect of sucrose, glucose, fructose, and galactose on the efficiency of the process was investigated. The optimal concentration of sucrose in the medium and replenishment was determined. The influence of peptone, tryptone, and casein hydrolysate of various brands on the effectiveness of the fermentation was analyzed. The optimal concentrations of peptone and yeast extract in the medium and replenishment were determined. As a result, owing to the optimization, the process of fermentation was simplified, a new composition of a complex replenishment was designed, and sucrose-specific consumption was reduced by two times, whereas the expenses of peptone and yeast extract were decreased by about 2.5 times, the yield of biomass per unit of the culture broth volume grew by ∼ 40%, and that of the protein of interest increased by ∼ 60%.