Different Modes of Interaction in Cyanobacterial Complexes of Plastocyanin and Cytochrome f

The highly efficient electron-transfer chain in photosynthesis demonstrates a remarkable variation among organisms in the type of interactions between the soluble electron-transfer protein plastocyanin and it partner cytochrome f. The complex from the cyanobacterium Nostoc sp. PCC 7119 was studied u...

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Bibliographic Details
Published in:Biochemistry (Easton) 2005-03, Vol.44 (9), p.3176-3183
Main Authors: Díaz-Moreno, Irene, Díaz-Quintana, Antonio, De la Rosa, Miguel A, Crowley, Peter B, Ubbink, Marcellus
Format: Article
Language:English
Subjects:
Cyanobacteria - chemistry
Cyanobacteria - enzymology
Cyanobacteria - metabolism
Cytochromes f - chemistry
Cytochromes f - metabolism
Hydrophobic and Hydrophilic Interactions
Models, Molecular
Multiprotein Complexes - chemistry
Multiprotein Complexes - metabolism
Nostoc - enzymology
Nuclear Magnetic Resonance, Biomolecular
Osmolar Concentration
Plastocyanin - chemistry
Plastocyanin - metabolism
Protein Binding
Static Electricity
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Summary:The highly efficient electron-transfer chain in photosynthesis demonstrates a remarkable variation among organisms in the type of interactions between the soluble electron-transfer protein plastocyanin and it partner cytochrome f. The complex from the cyanobacterium Nostoc sp. PCC 7119 was studied using nuclear magnetic resonance spectroscopy and compared to that of the cyanobacterium Phormidium laminosum. In both systems, the main site of interaction on plastocyanin is the hydrophobic patch. However, the interaction in the Nostoc complex is highly dependent on electrostatics, contrary to that of Phormidium, resulting in a binding constant that is an order of magnitude larger at low ionic strength for the Nostoc complex. Studies of the mixed complexes show that these differences in interactions are mainly attributable to the surface properties of the plastocyanins.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi047855z