Placental expression of the insulin receptor binding protein GRB10 : Relation to human fetoplacental growth and fetal gender

Abstract Introduction Imprinted genes play an important role in mammalian fetoplacental growth and development. We have evaluated whether the placental expression of two imprinted genes, growth factor receptor-binding protein 10 ( GRB10 ) and pleckstrin homology-like domain, family A, member 2 ( PHL...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2015-11, Vol.36 (11), p.1225-1230
Main Authors: Mukhopadhyay, A, Ravikumar, G, Dwarkanath, P, Meraaj, H, Thomas, A, Crasta, J, Thomas, T, Kurpad, A.V, Sridhar, T.S
Format: Article
Language:English
Subjects:
Adolescent
Adult
Cohort Studies
Female
Fetal growth restriction
Fetal Growth Retardation - metabolism
Gender
GRB10
GRB10 Adaptor Protein - metabolism
Human placenta
Humans
Imprinted genes
Infant, Newborn
Infant, Small for Gestational Age
Internal Medicine
Male
Obstetrics and Gynecology
Placenta - metabolism
Pregnancy
Sex Factors
SGA
Young Adult
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Summary:Abstract Introduction Imprinted genes play an important role in mammalian fetoplacental growth and development. We have evaluated whether the placental expression of two imprinted genes, growth factor receptor-binding protein 10 ( GRB10 ) and pleckstrin homology-like domain, family A, member 2 ( PHLDA2 ) correlate with human fetoplacental growth parameters. Methods Placentae (n = 77) were collected from small- (SGA) and appropriate- (AGA) for gestational age full-term singleton pregnancies (n = 36 SGA and 41 AGA). Placentae and neonates were weighed at birth. Realtime quantitative PCR was performed to assess placental transcript abundance of GRB10 and PHLDA2 normalized to a panel of reference genes. Results Placental GRB10 transcript abundance associated positively with placental weight (r = 0.307, P  = 0.007), birth weight (r = 0.267, P  = 0.019) and neonatal head circumference (r = 0.280, P  = 0.014). Placental GRB10 transcript levels were significantly lower in male SGA placentae compared to the male AGA placentae. Placental PHLDA2 transcript abundance did not show any associations with maternal, placental or neonatal parameters. Discussion Placental GRB10 expression was found to be associated positively with placental weight, birth weight, and neonatal head circumference, especially in males. Hence, we speculate that placental GRB10 plays a role in regulating fetoplacental growth and thereby in the pathophysiology of fetal growth restriction in the context of fetal gender.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2015.09.006