Identification of a functionally important amino acid residue near to the amino-terminus of the human immunodeficiency virus type 1 Vif protein

Previous studies have reported the importance of residues in the central and carboxy-terminal regions of HIV-1 Vif as being important for a Vif+ phenotype. Mutants in a residue (Trp21) near to the amino-terminus of Vif, which is conserved between HIV-1, HIV-2, and SIVmac, were generated in the permi...

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Bibliographic Details
Published in:Virus genes 1999, Vol.19 (1), p.15-22
Main Authors: Boyce, M, Willingmann, P, McCrae, M
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Line
DNA Mutational Analysis
DNA, Recombinant - genetics
Gene Expression
Gene Products, vif - genetics
HIV-1 - genetics
HIV-1 - growth & development
Human immunodeficiency virus 1
Humans
Mutagenesis, Site-Directed
Phenotype
vif Gene Products, Human Immunodeficiency Virus
Virus Replication
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Summary:Previous studies have reported the importance of residues in the central and carboxy-terminal regions of HIV-1 Vif as being important for a Vif+ phenotype. Mutants in a residue (Trp21) near to the amino-terminus of Vif, which is conserved between HIV-1, HIV-2, and SIVmac, were generated in the permissive C8166 cell line, and their Vif phenotype determined by monitoring their growth on the nonpermissive H9 cell line. Of seven variants at position 21 of Vif only phenylalanine substituted successfully for Trp21. The expression of the mutant Vif proteins was shown to be similar to wildtype levels, in C8166 cells, in all cases except one. These data identify Trp21 as an amino-terminal residue which is important to Vif function, in allowing growth of virus in nonpermissive H9 cells, and suggest that there is limited scope for variation at this position of HIV-1 Vif.
ISSN:0920-8569
1572-994X
DOI:10.1023/A:1008180421245