The Duality of Fish Gonadotropin Receptors: Cloning and Functional Characterization of a Second Gonadotropin Receptor cDNA Expressed in the Ovary and Testis of Amago Salmon (Oncorhynchus rhodurus)

We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. T...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-11, Vol.265 (2), p.366-371
Main Authors: Oba, Yuichi, Hirai, Toshiaki, Yoshiura, Yasutoshi, Yoshikuni, Michiyasu, Kawauchi, Hiroshi, Nagahama, Yoshitaka
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Brackish
Cloning, Molecular
COS Cells
Cyclic AMP - biosynthesis
DNA Primers - genetics
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Female
Freshwater
Gene Expression
Humans
Male
Marine
Molecular Sequence Data
Oncorhynchus - genetics
Oncorhynchus - metabolism
Oncorhynchus rhodurus
Ovary - metabolism
Phylogeny
Receptors, Gonadotropin - genetics
Receptors, Gonadotropin - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Testis - metabolism
Tissue Distribution
Transfection
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Summary:We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. The highest homology occurs with mammalian FSH receptors (49%). Functional characterization examined in COS-7 cells transiently transfected with sGTH-RI showed the largest increase in cAMP production when exposed to salmon GTH I. These results provide the first evidence in any ectothermic vertebrate, the amago salmon, of the duality of gonadotropin receptors.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1700