Design of division specific primers of Ralstonia solanacearum and application to the identification of European isolates

A PCR diagnostic test for detection of Ralstonia solanacearum at the infraspecific level was developed, based on polymorphisms within the 16S rRNA gene sequence. Partial sequences of this gene were determined for three French isolates which showed the characteristics of R. solanacearum subdivision 2...

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Bibliographic Details
Published in:European journal of plant pathology 1999-06, Vol.105 (4), p.373-380
Main Authors: Boudazin, G. (Laboratoire de Pathologie Vegetale INRA, Beaucouze (France).), Roux, A.C. Le, Josi, K, Labarre, P, Jouan, B
Format: Article
Language:English
Subjects:
ARN
Bacteria
Biological and medical sciences
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
EUROPA
EUROPE
Fundamental and applied biological sciences. Psychology
GENE
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
GENES
Microbiology
PCR
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
PSEUDOMONAS SOLANACEARUM
Ralstonia solanacearum
RNA
Techniques used in virology
Virology
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Summary:A PCR diagnostic test for detection of Ralstonia solanacearum at the infraspecific level was developed, based on polymorphisms within the 16S rRNA gene sequence. Partial sequences of this gene were determined for three French isolates which showed the characteristics of R. solanacearum subdivision 2a described by Taghavi et al. (1996). Oligonucleotide primers were designed to incorporate the nucleotide triplet (at positions 458-460 of the 16S rRNA gene) which differs between divisions 1 and 2 16S rRNA sequences of R. solanacearum isolates. A simple PCR test unambiguously revealed the division of each isolate. The PCR test was useful for identification of isolates of R. solanacearum from Europe.
ISSN:0929-1873
1573-8469
DOI:10.1023/A:1008763111230