Complementation cloning of salt tolerance determinants from the marine hyphomycete Dendryphiella salina in Aspergillus nidulans

A preliminary physiological characterization of osmoregulation in an Aspergillus nidulans salt sensitive mutant carrying cotransformed DNA sequences from the marine hyphomycete Dendryphiella salina is described. A genomic DNA library made in lambda EMBL3 was cotransformed with the plasmid pDJB3 into...

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Bibliographic Details
Published in:Mycological research 1999-10, Vol.103 (10), p.1252-1258
Main Authors: CLEMENT, D. J., STANLEY, M. S., O'NEIL, J., WOODCOCK, N. A., FINCHAM, D. A., CLIPSON, N. J. W., HOOLEY, P.
Format: Article
Language:English
Subjects:
arginine
Aspergillus nidulans
Aspergillus nidulellus
Biological and medical sciences
biosynthesis
Dendryphiella salina
Deuteromycotina
Fundamental and applied biological sciences. Psychology
genetic complementation
genetic markers
genetic transformation
genetically modified organisms
genotype
growth rate
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
marine fungi
Microbiology
mutants
mutation
Mycology
nucleotide sequences
polyols
salt tolerance
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Summary:A preliminary physiological characterization of osmoregulation in an Aspergillus nidulans salt sensitive mutant carrying cotransformed DNA sequences from the marine hyphomycete Dendryphiella salina is described. A genomic DNA library made in lambda EMBL3 was cotransformed with the plasmid pDJB3 into an A. nidulans host carrying a selectable marker for transformation (pyrG89) and a salt sensitivity mutation (sltA1). In some cases D. salina clones appeared to directly complement the salt sensitivity mutation by restoring salt and arginine insensitivity. Other clones gave marked increases in salt tolerance and yet the hosts remained arginine sensitive. Improvements in salt tolerance correlated with an enhanced ability to induce polyol biosynthesis during salt challenge.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756299008424