N(6)-Furfuryladenine, kinetin, protects against Fenton reaction-mediated oxidative damage to DNA

N(6)-Furfuryladenine (kinetin) has been shown to have anti-ageing effects on several different systems including plants, human cells in culture, and fruitflies. Since most of the experimental data point toward kinetin acting as an antioxidant both in vitro and in vivo, and since much evidence suppor...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-11, Vol.265 (2), p.499-502
Main Authors: Olsen, A, Siboska, G E, Clark, B F, Rattan, S I
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
Animals
Antioxidants - pharmacology
Cattle
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
DNA - drug effects
DNA - metabolism
DNA Damage
Free Radical Scavengers - pharmacology
Humans
Hydrogen Peroxide - metabolism
Hydrogen Peroxide - toxicity
In Vitro Techniques
Kinetin
Models, Biological
Oxidation-Reduction
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:N(6)-Furfuryladenine (kinetin) has been shown to have anti-ageing effects on several different systems including plants, human cells in culture, and fruitflies. Since most of the experimental data point toward kinetin acting as an antioxidant both in vitro and in vivo, and since much evidence supporting a causal role of oxidative damage in ageing is accumulating, we tested the antioxidant properties of kinetin directly. Using 8-oxo-2'deoxyguanosine (8-oxo-dG) in calf thymus DNA as a marker for oxidative damage, we demonstrate that kinetin significantly (P < 0.005) protects the DNA against oxidative damage mediated by the Fenton reaction. Kinetin inhibited 8-oxo-dG formation in a dose-dependent manner with a maximum of 50% protection observed at 100 microM kinetin.
ISSN:0006-291X
DOI:10.1006/bbrc.1999.1669