Biodegradation of 3-Chloro-1,2-propanediol with Saccharomyces cerevisiae

• Published in: Journal of agricultural and food chemistry 2004-10, Vol.52 (20), p.6165-6169
• Main Authors: Bel-Rhlid, Rachid, Talmon, Jean P, Fay, Laurent B, Juillerat, Marcel A
• Format: Article
• Language: English
• Subjects: alpha-Chlorohydrin - metabolism; Bioconversions. Hemisynthesis; biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Chlorides - metabolism; contaminants; detoxification (processing); food contamination; Fundamental and applied biological sciences. Psychology; Glucose - pharmacology; Hydrogen-Ion Concentration; Kinetics; metabolites; Methods. Procedures. Technologies; organochlorine compounds; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Stereoisomerism; yeasts
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf048980k

A novel enzymatic dehalogenating activity of 3-chloro-1,2-propanediol (3-MCPD) with Saccharomyces cerevisiae (baker's yeast) is reported. All bioconversion assays were carried out under aerobic conditions, at 28 °C, and the kinetics were monitored. The biodegradation was performed at different pH values (6.2, 7.0, and 8.2), in the presence and absence of glucose, using racemic 3-MCPD at two different concentrations (7.3 μmol/L and 27 mmol/L). Optimal conversion (68%) of racemic (R,S)-3-MCPD at a concentration of 27 mmol/L was achieved after 48 h of reaction time, at pH 8.2, and in the presence of glucose. At a concentration of 7.3 μmol/L, 73% degradation was observed after 72 h, at pH 8.2 and in the absence of glucose. Under the same experimental conditions, the conversion of pure (S)-3-MCPD (85%) was higher than that of the (R)-enantiomer (60%). Keywords: 3-Chloro-1,2-propanediol; biodegradation; Saccharomyces cerevisiae