The circadian clock-containing photoreceptor cells in Xenopus laevis express several isoforms of casein kinase I

• Published in: Brain research. Molecular brain research. 2005-05, Vol.136 (1), p.199-211
• Main Authors: Constance, Cara M., Fan, Jin-Yuan, Preuss, Fabian, Green, Carla B., Price, Jeffrey L.
• Format: Article
• Language: English
• Subjects: Alternative splicing; Animals; Autoradiography - methods; Biological and medical sciences; Blotting, Northern - methods; Blotting, Western - methods; Casein Kinase Idelta - genetics; Casein Kinase Idelta - metabolism; Casein Kinase Iepsilon - genetics; Casein Kinase Iepsilon - metabolism; Cell Count; Cell Line; Circadian Rhythm - physiology; Cloning, Molecular - methods; Cricetinae; Doubletime; Drosophila; Eye and associated structures. Visual pathways and centers. Vision; Fundamental and applied biological sciences. Psychology; Gene Expression - physiology; Gene Expression - radiation effects; Gene Library; Genetic Variation - physiology; Humans; In Situ Hybridization - methods; Mutagenesis - physiology; Nuclear localization; Period protein; Photoreceptor Cells - metabolism; Protein Isoforms - metabolism; Protein phosphorylation; Protein stability; Rats; Retina - cytology; Retina - physiology; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - biosynthesis; Subcellular Fractions - metabolism; Transfection - methods; Vertebrates: nervous system and sense organs; Xenopus laevis
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/j.molbrainres.2005.02.009

The frog ( Xenopus laevis) retina has been an important model for the analysis of retinal circadian rhythms. In this paper, several isoforms of X. laevis casein kinase I (CKI) were analyzed to address whether they are involved in the phosphorylation and degradation of period protein (PER), as they are in the circadian oscillators of other species. cDNAs encoding two splice variants of CKIδ (a full-length form and deletion isoform, which is missing an exon that encodes a putative nuclear localization signal and two evolutionarily conserved protein kinase domains) were isolated and analyzed, together with a previously isolated CKIε isoform. Both CKIδ and CKIε were shown to be constitutively expressed in the photoreceptors of the retina, where a circadian clock has been localized. Both the full-length CKIδ and CKIε were shown to have kinase activity in vitro, and the full-length CKIδ phosphorylated and degraded Drosophila PER when expressed in Drosophila S2 cells. The expression and biochemical characteristics of these CKIs are consistent with an evolutionarily conserved role for CKI in the Xenopus retinal clock. The CKIδ deletion isoform did not exhibit kinase activity and did not trigger degradation of PER. Subcellular localization of both CKIδ isoforms was cytoplasmic in several cell culture lines, but the full-length CKIδ, and not the deletion CKIδ isoform, was localized to both the nucleus and the cytoplasm in Drosophila S2 cells. These results indicate that the sequences missing in the deletion CKIδ isoform are important for the nuclear localization and kinase activity of the full-length isoform and that one or both of these features are necessary for degradation of Drosophila PER.